Description
Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live C. vulgaris cells in a 3D projection rotating around the X axis. The cells were live and dark acclimed during the imaging. Part of the cells were untreated and part were treated with DCMU to form a mixture of both cell types. The fluorescence lifetime is differs between the cells in the field of view, depending on their exposure to DCMU.This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"
Date made available | 6 Mar 2024 |
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Publisher | King's College London |