Dr Chan obtained his MEng (2001) and PhD (EPSRC Industrial CASE AWARD 2004, Supervisor: Prof Sergei G. Kazarian) at Imperial College London. He then became a research associate in Prof Kazarian's group between 2004 and 2012 including time as an EPSRC Life Science Interface (LSI) fellow between 2006 and 2009. While an EPSRC LSI fellow, Dr Chan spent 1 year (2007) in Rutgers University, Newark, USA, with Prof Richard Mendelsohn's group as a visiting researcher. In 2012, Dr Chan acquired the Lectureship in Pharmaceutical Molecular Spectroscopy in the Institute of Pharmaceutical Science at King’s College London and in 2014, he joined as a committee member in the Joint Pharmaceutical and Analysis Group (JPAG).

Dr Chan has published over 60 peer-reviewed research articles and book chapters primarily in the development of novel applications of Fourier Transform Infrared (FTIR) spectroscopy for testing pharmaceutical and biological systems. Dr Chan has been awarded the Weinberg Prize in 2004 (Imperial College London, Department of Chemical Engineering) for his PhD and the Hounsfield poster prize in 2009 (Imperial College London) for his bio-spectroscopic research. Since gaining his lectureship at King’s, Dr Chan research has been supported by the Royal Society, EPSRC and the Diamond Light Source. He currently has an h-index of 29 according to the Web Of Knowledge.

You can follow updates from Dr Chan on Twitter @IResearch_KAC, Linkedin and Researchgate.  

2001-2012

In his early research, Dr Chan developed many new infrared spectroscopic imaging and Raman applications, particularly using the attenuated total reflection (ATR) FTIR imaging, to study a wide range of pharmaceutical and biological systems. These include label-free imaging of drug distributions in compacted pharmaceutical tablets, dissolutions of pharmaceutical formulations in water, stabilities of pharmaceutical substances under controlled environments in a high-throughput manner as well as diffusions of chemicals in polymer film/fibre and biological tissues such as hair and skin, composite materials, microfluidic systems and live cells. He has also developed expertise in applying confocal Raman to obtain depth profiles of samples and using tip-enhanced Raman to study carbon nanotubes.

2012-current

Recently, Dr Chan’s research is mainly focused on the application of FTIR to study how living cells interact with pharmaceutical substances.

Quantification of drug diffusion in live cells

Quantifying the rate and the amount of drug entering live cells is an essential part of the medicine development process. Infrared spectroscopy is a label-free, chemically selective tool for analysing the composition of live cells in culture that has the potential to quantify, in situ, the amount of drug entering living cells in a non-destructive manner.

However, the strong absorbance of water in parts of the mid-IR spectral regions is one of the main barriers for using FTIR spectroscopy to study living cells due to:

1)      Live cells are often bathed in medium which makes FTIR measurement very difficult

2)      Live cells contains ~70% water

As a result, live cell measurements using FTIR often have limited sensitivity and were generally regarded as not suitable for quantifying drugs in live cells.

To improve the sensitivity and the detection limit of FTIR method for live cell measurements, such that it will be suitable to analyse some cancer drugs in living cell at therapeutic relevant level, Dr Chan’s laboratory has applied a multi bounce ATR method to optimised the path length and an optical filter to improve the efficiency in the collection of light. Using these novel adaptations it was possible to quantify 20 mM of fluorouracil (a widely used cancer drug) in cell culture medium using a standard FTIR instrument and it was possible to quantify and measure the flux of fluorouracil in situ in living cells treated with 80 micromolar of drug. These are therapeutic relevant concentration levels which were not detectable using standard FTIR approaches.

High resolution FTIR imaging of living cell 

Recently Dr Chan has been collaborating with scientists from the Diamond Light Source to explore the potential to image living cells at sub-wavelength spatial resolution. He uses solid immersion technique to increase the numerical aperture of exisiting objectives and combines it with high brighness synchrotron FTIR microscopic method to achieve high signal to noise, high resolution imaging. The advantage of this approach is that it allows the study of chemical components inside living cells at a sub-cellular level, which can help to better understand cellular biological process but it is a challenging task. More results will be published soon. 

In his early research, Dr Chan developed many new infrared spectroscopic imaging and Raman applications, particularly using the attenuated total reflection (ATR) FTIR imaging, to study a wide range of pharmaceutical and biological systems. These include label-free imaging of drug distributions in compacted pharmaceutical tablets, dissolutions of pharmaceutical formulations in water, stabilities of pharmaceutical substances under controlled environments in a high-throughput manner as well as diffusions of chemicals in polymer film/fibre and biological tissues such as hair and skin, composite materials, microfluidic systems and live cells. He has also developed expertise in applying confocal Raman to obtain depth profiles of samples and using tip-enhanced Raman to study carbon nanotubes. 

Recently, Dr Chan’s research is mainly focused on the application of FTIR to study how living cells interact with pharmaceutical substances and techniques to improve spatial resolution and specificity of FTIR.

You can follow updates from Dr Chan on Twitter @IResearch_KAC, Linkedin and Researchgate.

Travel grant

We would like to acknowledge the Analytical Chemistry Trust Fund (Royal Society of Chemistry) for their support of an overseas conference travel grant of £600 for student Helena Friedrich in 2023.