Research output per year
Research output per year
Personal profile
Research interests
The group is interested in the study of protein structure, dynamics and interactions in solution by NMR spectroscopy and other biophysical techniques supported by bioinformatics. The main areas of interest are muscle proteins involved in the regulation of muscle contraction in the sarcomere (MyBP-C, Calponin) as well as proteins regulation protein expression as a response to work load on muscle (MS1/ABRA); the regulation of kinase activity by regulatory domains in kinases (Nek2) as well as associated scaffolding proteins (Pi3K/Cin85, AuroraA/MSPS/TACC3); the relationship of sequence to structure in multidomain proteins where we have been recenrly identifying a number of new domains (MS1/ABRA, PC1) and have identified the precise nature of domain boundaries for others (Cin85). We are also investigating the use of solid state NMR spectroscopy for the study of filamentous protein assemblies that are vital for the function of muscle.
Research interests (short)
Structure, dynamics and interactions of signalling, scaffolding and muscle proteins using NMR spectroscopy and other biophysical methods
Click here for the Pfuhl group webpage
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Keywords
- QP Physiology
- NMR spectroscopy
- Protein structure
- Protein-Protein interactions
- Biophysics
- Protein dynamics
- Cardiac muscle
- Muscle regulation
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Collaborations and top research areas from the last five years
Recent external collaboration on country/territory level. Dive into details by clicking on the dots or
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Differential effects of mutations of POPDC proteins on heteromeric interaction and membrane trafficking
Swan, A. H., Schindler, R. F. R., Savarese, M., Mayer, I., Rinné, S., Bleser, F., Schänzer, A., Hahn, A., Sabatelli, M., Perna, F., Chapman, K., Pfuhl, M., Spivey, A. C., Decher, N., Udd, B., Tasca, G. & Brand, T., Dec 2023, In: Acta Neuropathologica Communications. 11, 1, 4.Research output: Contribution to journal › Article › peer-review
Open Access6 Citations (Scopus) -
Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays
Parijat, P., Ponnam, S., Attili, S., Campbell, K., El-Mezgueldi, M., Pfuhl, M. & Kampourakis, T., Dec 2023, In: Scientific Reports. 13, 1, 5216.Research output: Contribution to journal › Article › peer-review
Open AccessFile3 Citations (Scopus)70 Downloads (Pure) -
Obscurin Rho GEF domains are phosphorylated by MST-family kinases but do not exhibit nucleotide exchange factor activity towards Rho GTPases in vitro
Koch, D., Kho, A. L., Fukuzawa, A., Alexandrovich, A., Vanaanen, K. J., Beavil, A., Pfuhl, M., Rees, M. & Gautel, M., Apr 2023, In: PLoS ONE. 18, 4 April, e0284453.Research output: Contribution to journal › Article › peer-review
Open Access3 Citations (Scopus) -
Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
Rees, M., Nikoopour, R., Alexandrovich, A., Pfuhl, M., Lopes, L. R., Akhtar, M. M., Syrris, P., Elliott, P., Carr-White, G. & Gautel, M., Sept 2023, In: Journal of Structural Biology. 215, 3, 108009.Research output: Contribution to journal › Article › peer-review
Open Access -
East Coast fever, a neglected tropical disease with an outdated vaccine approach?
Kolakowski, J., Connelley, T., Lukacik, P., Pfuhl, M. & Werling, D., Nov 2022, In: TRENDS IN PARASITOLOGY. 38, 11, p. 930-932 3 p.Research output: Contribution to journal › Short survey › peer-review
Open Access4 Citations (Scopus)
Projects
- 18 Finished
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A cryo-electron microscope for structural biology (including single-particle and tomography) at KCL.
Conte, M., Atherton, J., Beavil, A., Bergeron, J., Booth, P., Burrone, J., Carlton, J., De Nicola, G., Ehler, E., Fleck, R., Fraternali, F., Garnett, J., Gautel, M., Isaacson, R., Kampourakis, T., Malim, M., McDonnell, J., Neil, S., Odendall, C., Pfuhl, M., Politis, A., Steiner, R. & Sutton, B.
BBSRC Biotechnology and Biological Sciences Research Council
1/08/2022 → 31/07/2023
Project: Research
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SECMALS: A dual DLS and SEC-MALS instrumentation to characterize the oligomerization of protein samples for structural biology, mechanistic biology, and therapeutic development
Bergeron, J., Atherton, J., Beavil, A., Booth, P., Conte, M., Garnett, J., Isaacson, R., Malim, M., Muller, M., Pfuhl, M., Politis, A., Sanderson, M., Steiner, R., Surman, A. & Sutton, B.
BBSRC Biotechnology and Biological Sciences Research Council
3/11/2021 → 31/10/2022
Project: Research
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UKRI CoA: Phosphodependent helix switches in cellular signalling
EPSRC Engineering and Physical Sciences Research Council
1/12/2020 → 30/09/2021
Project: Research
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Isothermal titration calorimetry instrumentation for structural biology, biological mechanisms and drug discovery
Conte, M., Gautel, M., McDonnell, J., Isaacson, R., M. Barry, S., Garnett, J., Steiner, R., Muller, M., Pfuhl, M., Bergeron, J., Malim, M., Naglik, J., Kampourakis, T., Burgoyne, J., Pastore, A. & De Nicola, G.
BBSRC Biotechnology and Biological Sciences Research Council
1/10/2020 → 30/09/2021
Project: Research
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Phosphodependent helix switches in cellular signalling
BBSRC Biotechnology and Biological Sciences Research Council
1/03/2019 → 28/02/2022
Project: Research
Activities
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EMBO Journal (Journal)
Mark Pfuhl (Editor)
2008 → …Activity: Publication peer-review and editorial work › Publication peer-review
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CCPN meeting session chair
Mark Pfuhl (Chair)
Aug 2006 → …Activity: Participating in or organising an event › Participation in conference
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Biochemical Society
Mark Pfuhl (Member)
2005 → …Activity: Other › Types of External academic engagement - Membership of external research organisation
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Journal of Molecular Biology (Journal)
Mark Pfuhl (Editor)
2002 → …Activity: Publication peer-review and editorial work › Publication peer-review