14-3-3 Coordinates Microtubules, Rac, and Myosin II to Control Cell Mechanics and Cytokinesis

Qiongqiong Zhou; Yee-Seir Kee; Christopher C. Poirier; Christine Jelinek; Jonathan Osborne; Srikanth Divi; Alexandra Surcel; Marie E. Will; Ulrike Eggert; Annette Mueller-Taubenberger; Pablo A. Iglesias; Robert J. Cotter; Douglas N. Robinson

doi:10.1016/j.cub.2010.09.048

14-3-3 Coordinates Microtubules, Rac, and Myosin II to Control Cell Mechanics and Cytokinesis

Qiongqiong Zhou, Yee-Seir Kee, Christopher C. Poirier, Christine Jelinek, Jonathan Osborne, Srikanth Divi, Alexandra Surcel, Marie E. Will, Ulrike Eggert, Annette Mueller-Taubenberger, Pablo A. Iglesias, Robert J. Cotter, Douglas N. Robinson

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system.

Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling.

Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes-cytokinesis cell shape change and cell mechanics.

Original language	English
Pages (from-to)	1881-1889
Number of pages	9
Journal	Current Biology
Volume	20
Issue number	21
DOIs	https://doi.org/10.1016/j.cub.2010.09.048
Publication status	Published - 9 Nov 2010

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@article{49f473fb7cdc4de19a728a5c361fa34f,

title = "14-3-3 Coordinates Microtubules, Rac, and Myosin II to Control Cell Mechanics and Cytokinesis",

abstract = "Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system.Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling.Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes-cytokinesis cell shape change and cell mechanics.",

author = "Qiongqiong Zhou and Yee-Seir Kee and Poirier, {Christopher C.} and Christine Jelinek and Jonathan Osborne and Srikanth Divi and Alexandra Surcel and Will, {Marie E.} and Ulrike Eggert and Annette Mueller-Taubenberger and Iglesias, {Pablo A.} and Cotter, {Robert J.} and Robinson, {Douglas N.}",

year = "2010",

month = nov,

day = "9",

doi = "10.1016/j.cub.2010.09.048",

language = "English",

volume = "20",

pages = "1881--1889",

journal = "Current Biology",

issn = "0960-9822",

publisher = "Elsevier B.V.",

number = "21",

}

TY - JOUR

T1 - 14-3-3 Coordinates Microtubules, Rac, and Myosin II to Control Cell Mechanics and Cytokinesis

AU - Zhou, Qiongqiong

AU - Kee, Yee-Seir

AU - Poirier, Christopher C.

AU - Jelinek, Christine

AU - Osborne, Jonathan

AU - Divi, Srikanth

AU - Surcel, Alexandra

AU - Will, Marie E.

AU - Eggert, Ulrike

AU - Mueller-Taubenberger, Annette

AU - Iglesias, Pablo A.

AU - Cotter, Robert J.

AU - Robinson, Douglas N.

PY - 2010/11/9

Y1 - 2010/11/9

N2 - Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system.Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling.Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes-cytokinesis cell shape change and cell mechanics.

AB - Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system.Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling.Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes-cytokinesis cell shape change and cell mechanics.

U2 - 10.1016/j.cub.2010.09.048

DO - 10.1016/j.cub.2010.09.048

M3 - Article

SN - 0960-9822

VL - 20

SP - 1881

EP - 1889

JO - Current Biology

JF - Current Biology

IS - 21

ER -

14-3-3 Coordinates Microtubules, Rac, and Myosin II to Control Cell Mechanics and Cytokinesis

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