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16/18 genotyping in triage of persistent human papillomavirus infections with negative cytology in the English cervical screening pilot

Research output: Contribution to journalArticle

Matejka Rebolj, Adam R Brentnall, Christopher Mathews, Karin Denton, Miles Holbrook, Tanya Levine, Alexandra Sargent, John Smith, John Tidy, Xenia Tyler, Henry Kitchener

Original languageEnglish
Pages (from-to)455–463
Number of pages9
JournalBJC: British Journal of Cancer
Issue number6
Early online date14 Aug 2019
Publication statusPublished - 10 Sep 2019


King's Authors


In the English pilot of primary cervical screening with high-risk human papillomavirus (HR-HPV), we exploited natural viral clearance over 24 months to minimise unnecessary referral of HR-HPV+ women with negative cytology. Three laboratories were permitted to use 16/18 genotyping to select women for referral at 12-month recall. We estimated the clinical impact of this early genotyping referral.

The observed numbers of women referred to colposcopy and with detected high-grade cervical intraepithelial neoplasia (CIN2+), and of women who did not attend early recall in the three laboratories were compared with those estimated to represent a situation without an early genotyping referral. The 95% confidence intervals (CI) for the differences between the protocols were calculated by using a parametric bootstrap.

Amongst 127,238 screened women, 16,097 (13%) had HR-HPV infections. The genotyping protocol required 5.9% (95% CI: 4.4–7.7) additional colposcopies and led to a detection of 1.2% additional CIN2+ (95% CI: 0.6–2.0), while 2.3% (95% CI: 2.1–2.5) fewer HR-HPV+/cytology− women did not attend the early recall compared with the non-genotyping protocol.

In a screening programme with high quality of triage cytology and high adherence to early recall,16/18 genotyping of persistent HPV infections does not substantially increase CIN2+ detection.

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