3D Super-Resolution Imaging with Blinking Quantum Dots

Yong Wang; Gilbert Fruhwirth; En Cai; Tony Ng; Paul R Selvin

doi:10.1021/nl4026665

3D Super-Resolution Imaging with Blinking Quantum Dots

Yong Wang, Gilbert Fruhwirth, En Cai, Tony Ng, Paul R Selvin

Research output: Contribution to journal › Article › peer-review

100 Citations (Scopus)

Abstract

Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.

Original language	English
Article number	N/A
Pages (from-to)	5233-5241
Number of pages	9
Journal	Nano Letters
Volume	13
Issue number	11
DOIs	https://doi.org/10.1021/nl4026665
Publication status	Published - 1 Nov 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/nl4026665

Cite this

@article{0fe9b2862537450c8f5c84d75a00e5f7,

title = "3D Super-Resolution Imaging with Blinking Quantum Dots",

abstract = "Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.",

author = "Yong Wang and Gilbert Fruhwirth and En Cai and Tony Ng and Selvin, {Paul R}",

year = "2013",

month = nov,

day = "1",

doi = "10.1021/nl4026665",

language = "English",

volume = "13",

pages = "5233--5241",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - 3D Super-Resolution Imaging with Blinking Quantum Dots

AU - Wang, Yong

AU - Fruhwirth, Gilbert

AU - Cai, En

AU - Ng, Tony

AU - Selvin, Paul R

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.

AB - Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.

U2 - 10.1021/nl4026665

DO - 10.1021/nl4026665

M3 - Article

C2 - 24093439

SN - 1530-6984

VL - 13

SP - 5233

EP - 5241

JO - Nano Letters

JF - Nano Letters

IS - 11

M1 - N/A

ER -

3D Super-Resolution Imaging with Blinking Quantum Dots

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this