King's College London

Research portal

α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3

Research output: Contribution to journalArticlepeer-review

Nikki R. Paul, Jennifer L. Allen, Anna Chapman, Maria Morlan-Mairal, Egor Zindy, Guillaume Jacquemet, Laura Fernandez del Ama, Nermina Ferizovic, David M. Green, Jonathan D. Howe, Elisabeth Ehler, Adam Hurlstone, Patrick T. Caswell

Original languageEnglish
Pages (from-to)1013-1031
Number of pages19
JournalJournal of Cell Biology
Issue number6
Accepted/In press31 Jul 2015
Published14 Sep 2015


King's Authors


Invasive migration in 3D extracellular matrix (ECM) is crucial to cancer metastasis, yet little is known of the molecular mechanisms that drive reorganization of the cytoskeleton as cancer cells disseminate in vivo. 2D Rac-driven lamellipodial migration is well understood, but how these features apply to 3D migration is not clear. We find that lamellipodia- like protrusions and retrograde actin flow are indeed observed in cells moving in 3D ECM. However, Rab-coupling protein (RCP)-driven endocytic recycling of α5β1 integrin enhances invasive migration of cancer cells into fibronectin- rich 3D ECM, driven by RhoA and filopodial spike-based protrusions, not lamellipodia. Furthermore, we show that actin spike protrusions are Arp2/3-independent. Dynamic actin spike assembly in cells invading in vitro and in vivo is regulated by Formin homology-2 domain containing 3 (FHOD3), which is activated by RhoA/ROCK, establishing a novel mechanism through which the RCP-α5β1 pathway reprograms the actin cytoskeleton to promote invasive migration and local invasion in vivo.

Download statistics

No data available

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454