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99m Tc-single domain PD-l1 antibody (NM01): a novel imaging biomarker for non-invasive myocardial PD-l1 expression

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Muhummad Nazir, Daniel Hughes, Hong Hoi Ting, Gitasha Chand, Damion Bailey, Victoria Gibson, Kathryn Adamson, Jessica Johnson, Alexander R Lyon, Gary Cook

Original languageEnglish
JournalHeart
Volume108(suppl_2)
DOIs
PublishedSep 2022

King's Authors

Abstract

Objective: Immune checkpoint inhibitors have revolutionized the care of cancer patients and involve blockade of the PD-L1 receptor. Myocardial PD-L1 expression has been implicated in mice models in myocarditis and autoimmune dilated cardiomyopathy. PD-L1 myocardial expression may have a potential role in immune checkpoint inhibitor myocarditis. We aimed to determine the non-invasive assessment of myocardial PD-L1 expression using 99mTc-single domain PD-L1 antibody (NM01) SPECT-CT.

Methods: Cancer patients (n=10) underwent two SPECT-CT scans, at baseline and following 9 weeks cancer therapy. Patients were administered with a novel radiotracer, 99mTc single domain PD-L1 antibody (NM-01), prior to imaging. Baseline and 9-week left ventricular to blood pool ratios (LVmax:BP) and right ventricular to blood pool ratios (RVmax:BP) were measured. Measurements were repeated following a three-month period.

Results: Myocardial PD-L1 expression was evident in all patients. Mean LVmax:BP values were 2.9±0.63 at baseline vs 2.60±0.82 at 9-week, (p=0.37). Mean RVmax:BP were 1.74±0.28 at baseline vs 1.68±0.51 at 9-week, (p=0.71). There was excellent reliability of measurements with intraclass correlation coefficient of 0.99 (95% confidence interval 0.94–0.99, p
Conclusion: We demonstrate the first in man study for a novel quantitative imaging biomarker for the assessment of PD-L1 expression of the heart. This finding will now facilitate future studies to allow us to determine the role of PD-L1 in a range of cardiac disease such as myocarditis and cardiomyopathies.

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