A comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems

G. Ekin Atilla-Gokcumen*, Ulrike S. Eggert

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Although lipids participate in many cellular processes both as signaling and structural molecules, our understanding of the roles of individual lipids as well as global changes in lipid composition are limited. Here we describe an LC-MS based method to identify lipids that change in a biological process. This method describes the isolation of lipids from tissue culture cells, sample preparation for LC-MS, the LC-MS run, and the subsequent data processing steps to compare the global lipid profiles and identify species that are enhanced or depleted. Identifying lipids that change is the first step towards functional studies to unravel their roles.

Original languageEnglish
Title of host publicationMethods in Membrane Lipids
Subtitle of host publicationSecond Edition
PublisherSpringer New York LLC
Pages103-112
Number of pages10
ISBN (Electronic)9781493917525
ISBN (Print)9781493917518
DOIs
Publication statusPublished - 20 Oct 2014

Keywords

  • LC-MS based lipid profiling
  • Lipids
  • Untargeted lipid profiling

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