A determination of the pre-analytical storage conditions for insulin like growth factor-I and type III procollagen peptide

Richard I. G. Holt; Ioulietta Erotokritou-Mulligan; Sean A. Ridley; Cathy M. McHugh; E. Eryl Bassett; David A. Cowan; Christiaan Bartlett; Peter H. Soenksen

doi:10.1016/j.ghir.2008.06.001

A determination of the pre-analytical storage conditions for insulin like growth factor-I and type III procollagen peptide

Richard I. G. Holt, Ioulietta Erotokritou-Mulligan, Sean A. Ridley, Cathy M. McHugh, E. Eryl Bassett, David A. Cowan, Christiaan Bartlett, Peter H. Soenksen

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Objective: IGF-I and type III procollagen (P-III-P) have been proposed as markers to detect GH abuse. This study aims to determine whether the pre-analytical storage temperature or delayed centrifugation affect the measured IGF-I and P-III-P concentrations. Design: Observational study. Setting: Wellcome Trust Clinical Research Facility, Southampton. Subjects: Nineteen healthy volunteers. Intervention: Blood was collected into bottles containing a clotting agent, lithium heparin or EDTA. One sample from each group was centrifuged and stored at -80 degrees C (control sample). The remaining samples from each group were stored as either serum or whole blood at 4 degrees C or room temperature for up to five days prior to storage at -80 degrees C. Outcome measures: IGF-I and P-III-P. Results: The storage temperature or timing of centrifugation did not appear to affect IGF-I concentration. In contrast, the Measured P-III-P concentration rose by 6.5-7% per day in clotted and lithium heparin samples when stored as whole blood (p <0.006) or serum (6.2-6.5%, per day) at room temperature (p <0.001), P-III-P did not change when the samples were stored at 4 degrees C. Although collection into EDTA inhibited the rise in P-III-P, the baseline measured values were significantly higher than in other media and spiking experiments demonstrated that EDTA exerted a significant matrix effect on the assay. Conclusion: While the optimum collection method is immediate centrifugation and storage at -80 degrees C, it would seem acceptable to store serum or clotted blood samples at 4 degrees C, but not ambient temperature, for LIP to five days. It is incumbent on the anti-doping authorities to provide facilities to allow this. (C) 2008 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	43 - 50
Number of pages	8
Journal	GROWTH HORMONE AND IGF RESEARCH
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/j.ghir.2008.06.001
Publication status	Published - 2009

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ghir.2008.06.001

Cite this

@article{2bd6744f046548e1a2179fe92173d1e7,

title = "A determination of the pre-analytical storage conditions for insulin like growth factor-I and type III procollagen peptide",

abstract = "Objective: IGF-I and type III procollagen (P-III-P) have been proposed as markers to detect GH abuse. This study aims to determine whether the pre-analytical storage temperature or delayed centrifugation affect the measured IGF-I and P-III-P concentrations. Design: Observational study. Setting: Wellcome Trust Clinical Research Facility, Southampton. Subjects: Nineteen healthy volunteers. Intervention: Blood was collected into bottles containing a clotting agent, lithium heparin or EDTA. One sample from each group was centrifuged and stored at -80 degrees C (control sample). The remaining samples from each group were stored as either serum or whole blood at 4 degrees C or room temperature for up to five days prior to storage at -80 degrees C. Outcome measures: IGF-I and P-III-P. Results: The storage temperature or timing of centrifugation did not appear to affect IGF-I concentration. In contrast, the Measured P-III-P concentration rose by 6.5-7% per day in clotted and lithium heparin samples when stored as whole blood (p <0.006) or serum (6.2-6.5%, per day) at room temperature (p <0.001), P-III-P did not change when the samples were stored at 4 degrees C. Although collection into EDTA inhibited the rise in P-III-P, the baseline measured values were significantly higher than in other media and spiking experiments demonstrated that EDTA exerted a significant matrix effect on the assay. Conclusion: While the optimum collection method is immediate centrifugation and storage at -80 degrees C, it would seem acceptable to store serum or clotted blood samples at 4 degrees C, but not ambient temperature, for LIP to five days. It is incumbent on the anti-doping authorities to provide facilities to allow this. (C) 2008 Elsevier Ltd. All rights reserved.",

author = "Holt, {Richard I. G.} and Ioulietta Erotokritou-Mulligan and Ridley, {Sean A.} and McHugh, {Cathy M.} and Bassett, {E. Eryl} and Cowan, {David A.} and Christiaan Bartlett and Soenksen, {Peter H.}",

year = "2009",

doi = "10.1016/j.ghir.2008.06.001",

language = "English",

volume = "19",

pages = "43 -- 50",

journal = "GROWTH HORMONE AND IGF RESEARCH",

publisher = "Churchill Livingstone",

number = "1",

}

TY - JOUR

T1 - A determination of the pre-analytical storage conditions for insulin like growth factor-I and type III procollagen peptide

AU - Holt, Richard I. G.

AU - Erotokritou-Mulligan, Ioulietta

AU - Ridley, Sean A.

AU - McHugh, Cathy M.

AU - Bassett, E. Eryl

AU - Cowan, David A.

AU - Bartlett, Christiaan

AU - Soenksen, Peter H.

PY - 2009

Y1 - 2009

N2 - Objective: IGF-I and type III procollagen (P-III-P) have been proposed as markers to detect GH abuse. This study aims to determine whether the pre-analytical storage temperature or delayed centrifugation affect the measured IGF-I and P-III-P concentrations. Design: Observational study. Setting: Wellcome Trust Clinical Research Facility, Southampton. Subjects: Nineteen healthy volunteers. Intervention: Blood was collected into bottles containing a clotting agent, lithium heparin or EDTA. One sample from each group was centrifuged and stored at -80 degrees C (control sample). The remaining samples from each group were stored as either serum or whole blood at 4 degrees C or room temperature for up to five days prior to storage at -80 degrees C. Outcome measures: IGF-I and P-III-P. Results: The storage temperature or timing of centrifugation did not appear to affect IGF-I concentration. In contrast, the Measured P-III-P concentration rose by 6.5-7% per day in clotted and lithium heparin samples when stored as whole blood (p <0.006) or serum (6.2-6.5%, per day) at room temperature (p <0.001), P-III-P did not change when the samples were stored at 4 degrees C. Although collection into EDTA inhibited the rise in P-III-P, the baseline measured values were significantly higher than in other media and spiking experiments demonstrated that EDTA exerted a significant matrix effect on the assay. Conclusion: While the optimum collection method is immediate centrifugation and storage at -80 degrees C, it would seem acceptable to store serum or clotted blood samples at 4 degrees C, but not ambient temperature, for LIP to five days. It is incumbent on the anti-doping authorities to provide facilities to allow this. (C) 2008 Elsevier Ltd. All rights reserved.

AB - Objective: IGF-I and type III procollagen (P-III-P) have been proposed as markers to detect GH abuse. This study aims to determine whether the pre-analytical storage temperature or delayed centrifugation affect the measured IGF-I and P-III-P concentrations. Design: Observational study. Setting: Wellcome Trust Clinical Research Facility, Southampton. Subjects: Nineteen healthy volunteers. Intervention: Blood was collected into bottles containing a clotting agent, lithium heparin or EDTA. One sample from each group was centrifuged and stored at -80 degrees C (control sample). The remaining samples from each group were stored as either serum or whole blood at 4 degrees C or room temperature for up to five days prior to storage at -80 degrees C. Outcome measures: IGF-I and P-III-P. Results: The storage temperature or timing of centrifugation did not appear to affect IGF-I concentration. In contrast, the Measured P-III-P concentration rose by 6.5-7% per day in clotted and lithium heparin samples when stored as whole blood (p <0.006) or serum (6.2-6.5%, per day) at room temperature (p <0.001), P-III-P did not change when the samples were stored at 4 degrees C. Although collection into EDTA inhibited the rise in P-III-P, the baseline measured values were significantly higher than in other media and spiking experiments demonstrated that EDTA exerted a significant matrix effect on the assay. Conclusion: While the optimum collection method is immediate centrifugation and storage at -80 degrees C, it would seem acceptable to store serum or clotted blood samples at 4 degrees C, but not ambient temperature, for LIP to five days. It is incumbent on the anti-doping authorities to provide facilities to allow this. (C) 2008 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.ghir.2008.06.001

DO - 10.1016/j.ghir.2008.06.001

M3 - Article

VL - 19

SP - 43

EP - 50

JO - GROWTH HORMONE AND IGF RESEARCH

JF - GROWTH HORMONE AND IGF RESEARCH

IS - 1

ER -