A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

Giulia Villari; Asier Jayo; Jennifer Zanet; Briana Fitch; Bryan Serrels; Margaret Frame; Brian M. Stramer; Benjamin T. Goult; Maddy Parsons

doi:10.1242/jcs.175760

A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

Giulia Villari, Asier Jayo, Jennifer Zanet, Briana Fitch, Bryan Serrels, Margaret Frame, Brian M. Stramer, Benjamin T. Goult, Maddy Parsons^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown.Here,we showfor the first timethat fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascindependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on newnon actindependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease.

Original language	English
Pages (from-to)	4601-4614
Number of pages	14
Journal	Journal of Cell Science
Volume	128
Issue number	24
DOIs	https://doi.org/10.1242/jcs.175760
Publication status	Published - 2015

Keywords

Actin
Cytoskeleton
Fascin
Focal adhesion
Focal adhesion kinase
Microtubule
Migration

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1242/jcs.175760

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title = "A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration",

abstract = "Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown.Here,we showfor the first timethat fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascindependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on newnon actindependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease.",

keywords = "Actin, Cytoskeleton, Fascin, Focal adhesion, Focal adhesion kinase, Microtubule, Migration",

author = "Giulia Villari and Asier Jayo and Jennifer Zanet and Briana Fitch and Bryan Serrels and Margaret Frame and Stramer, {Brian M.} and Goult, {Benjamin T.} and Maddy Parsons",

year = "2015",

doi = "10.1242/jcs.175760",

language = "English",

volume = "128",

pages = "4601--4614",

journal = "Journal of Cell Science",

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T1 - A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

AU - Villari, Giulia

AU - Jayo, Asier

AU - Zanet, Jennifer

AU - Fitch, Briana

AU - Serrels, Bryan

AU - Frame, Margaret

AU - Stramer, Brian M.

AU - Goult, Benjamin T.

AU - Parsons, Maddy

PY - 2015

Y1 - 2015

N2 - Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown.Here,we showfor the first timethat fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascindependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on newnon actindependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease.

AB - Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown.Here,we showfor the first timethat fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascindependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on newnon actindependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease.

KW - Actin

KW - Cytoskeleton

KW - Fascin

KW - Focal adhesion

KW - Focal adhesion kinase

KW - Microtubule

KW - Migration

UR - http://www.scopus.com/inward/record.url?scp=84957640598&partnerID=8YFLogxK

U2 - 10.1242/jcs.175760

DO - 10.1242/jcs.175760

M3 - Article

AN - SCOPUS:84957640598

SN - 0021-9533

VL - 128

SP - 4601

EP - 4614

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 24

ER -

A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

Abstract

Keywords

UN SDGs

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