A discordance between the human muscle NCAM sequence and those seen in other NCAM cDNAs

TY - JOUR

T1 - A discordance between the human muscle NCAM sequence and those seen in other NCAM cDNAs

AU - Rossell, R J

AU - Malik, Afshan

AU - Helfrich, W

AU - Kemshead, J T

AU - Patel , K

PY - 1992/2/28

Y1 - 1992/2/28

N2 - A cDNA clone, designated NC7, has been isolated from human foetal kidney that partially codes for the 140 kDa isoform of human NCAM. This clone contains a 6 bp insert that is not present in the human muscle cDNA clone λ4.4. This same sequence has also been found in both a cDNA clone obtained from a human Small Cell Lung Carcinoma (SCLC) line and in human genomic DNA. Furthermore, an equivalent sequence to the 6 bp region identified in the above samples is present in mouse, rat and chicken NCAM. The 6 bp insertion does not lie at a predicted intron/exon boundary as extrapolated by homology studies with the chicken and, therefore, the mechanism by which the sequence is deleted from the human muscle clone λ4.4 remains unclear.

AB - A cDNA clone, designated NC7, has been isolated from human foetal kidney that partially codes for the 140 kDa isoform of human NCAM. This clone contains a 6 bp insert that is not present in the human muscle cDNA clone λ4.4. This same sequence has also been found in both a cDNA clone obtained from a human Small Cell Lung Carcinoma (SCLC) line and in human genomic DNA. Furthermore, an equivalent sequence to the 6 bp region identified in the above samples is present in mouse, rat and chicken NCAM. The 6 bp insertion does not lie at a predicted intron/exon boundary as extrapolated by homology studies with the chicken and, therefore, the mechanism by which the sequence is deleted from the human muscle clone λ4.4 remains unclear.

M3 - Article

SN - 0006-3002

VL - 1130

SP - 95

EP - 96

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

IS - 1

M1 - N/A

ER -