A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

J. Trägårdh; J. Schniete; M. Parsons; G. McConnell

doi:10.1063/1.4962207

A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

J. Trägårdh, J. Schniete, M. Parsons, G. McConnell

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Abstract

We demonstrate a femtosecond single pass Raman generator based on an YVO₄ crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broadband spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.

Original language	English
Article number	091303
Journal	APL Photonics
Volume	1
Issue number	9
DOIs	https://doi.org/10.1063/1.4962207
Publication status	Published - 1 Dec 2016

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A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

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