A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation

Rosalind H Morley, Kim Lachani, Damien Keefe, Michael J Gilchrist, Paul Flicek, James C Smith, Fiona C Wardle

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
Original languageEnglish
Pages (from-to)3829 - 3834
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number10
DOIs
Publication statusPublished - 10 Mar 2009

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