A gene regulatory network for mesoderm directed by zebrafish No tail

Rosie Morley; Kim Lachani; Damien Keefe; Andrew Nelson; Michael J Gilchrist; Paul Flicek; Jim Smith; Fiona C Wardle

doi:10.1016/j.mod.2009.06.771

A gene regulatory network for mesoderm directed by zebrafish No tail

Rosie Morley
, Kim Lachani
, Damien Keefe
, Andrew Nelson
, Michael J Gilchrist
, Paul Flicek
, Jim Smith
, Fiona C Wardle

Research output: Contribution to journal › Meeting abstract

Abstract

Using chromatin immunoprecipitation combined with genomic microarrays and massively parallel sequencing we have identified targets of No tail a (Ntla), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We have found that Ntl regulates a downstream network of other transcription factors and we identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. Using these data we have begun to assemble a Gene Regulatory Network to describe mesoderm formation and patterning in the early zebrafish embryo.

Original language	English
Pages (from-to)	S285 - S286
Journal	Mechanisms of Development
Volume	126
DOIs	https://doi.org/10.1016/j.mod.2009.06.771
Publication status	Published - 2009
Event	16th International Society of Developmental Biologists Congress 2009 - Edinburgh Duration: 10 Sept 2009 → 6 Sept 2010

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@article{3d5a5402b59243c8a3a08106b78e087b,

title = "A gene regulatory network for mesoderm directed by zebrafish No tail",

abstract = "Using chromatin immunoprecipitation combined with genomic microarrays and massively parallel sequencing we have identified targets of No tail a (Ntla), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We have found that Ntl regulates a downstream network of other transcription factors and we identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. Using these data we have begun to assemble a Gene Regulatory Network to describe mesoderm formation and patterning in the early zebrafish embryo.",

author = "Rosie Morley and Kim Lachani and Damien Keefe and Andrew Nelson and Gilchrist, \{Michael J\} and Paul Flicek and Jim Smith and Wardle, \{Fiona C\}",

year = "2009",

doi = "10.1016/j.mod.2009.06.771",

language = "English",

volume = "126",

pages = "S285 -- S286",

journal = "Mechanisms of Development",

publisher = "John Wiley \& Sons Inc.",

note = "16th International Society of Developmental Biologists Congress 2009 ; Conference date: 10-09-2009 Through 06-09-2010",

}

TY - JOUR

T1 - A gene regulatory network for mesoderm directed by zebrafish No tail

AU - Morley, Rosie

AU - Lachani, Kim

AU - Keefe, Damien

AU - Nelson, Andrew

AU - Gilchrist, Michael J

AU - Flicek, Paul

AU - Smith, Jim

AU - Wardle, Fiona C

PY - 2009

Y1 - 2009

N2 - Using chromatin immunoprecipitation combined with genomic microarrays and massively parallel sequencing we have identified targets of No tail a (Ntla), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We have found that Ntl regulates a downstream network of other transcription factors and we identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. Using these data we have begun to assemble a Gene Regulatory Network to describe mesoderm formation and patterning in the early zebrafish embryo.

AB - Using chromatin immunoprecipitation combined with genomic microarrays and massively parallel sequencing we have identified targets of No tail a (Ntla), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We have found that Ntl regulates a downstream network of other transcription factors and we identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. Using these data we have begun to assemble a Gene Regulatory Network to describe mesoderm formation and patterning in the early zebrafish embryo.

U2 - 10.1016/j.mod.2009.06.771

DO - 10.1016/j.mod.2009.06.771

M3 - Meeting abstract

VL - 126

SP - S285 - S286

JO - Mechanisms of Development

JF - Mechanisms of Development

T2 - 16th International Society of Developmental Biologists Congress 2009

Y2 - 10 September 2009 through 6 September 2010

ER -

A gene regulatory network for mesoderm directed by zebrafish No tail

Abstract

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