@inbook{e9f4edfc0e164a2790c10bfded2d9598,
title = "A high-content screening platform utilizing polarization anisotropy and FLIM microscopy - art. no. 685919",
abstract = "An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Forster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simultaneously capture the parallel and perpendicular components of both eGFP and mRFP1 fluorescence emission to provide a high-speed measurement of acceptor depolarization. Donor excited state lifetime measurements performed using laser scanning microscopy is then used to determine the FRET efficiency in a particular assay. A proof-of-principle assay is performed using mutant Jurkat human T-cells to illustrate the process by which FRET is first identified and then quantified by our high-content screening system",
author = "Matthews, \{D R\} and Ameer-Beg, \{S M\} and P Barber and Pierce, \{G P\} and Newman, \{R G\} and B Vojnovic and Carlin, \{L M\} and Keppler, \{M D\} and T Ng and K Suhling and M Irving and Farkas, \{D L\} and Nicolau, \{D V\} and Leif, \{R C\}",
year = "2008",
language = "English",
isbn = "0277-786X",
series = "PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)",
publisher = "SPIE - INT SOC OPTICAL ENGINEERING",
pages = "85919 -- 85919",
booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues Vi",
note = "Conference on Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues VI ; Conference date: 01-01-2008",
}