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A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation

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A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation. / Yang, Junyao; Moraga, Ana; Xu, Jing; Zhao, Yue; Luo, Peiyi; Lao, Ka Hou; Margariti, Andriana; Zhao, Qiang; Ding, Wei; Wang, Gang; Zhang, Min; Zheng, Lei; Zhang, Zhongyi; Hu, Yanhua; Wang, Wen; Shen, Lisong; Smith, Alberto; Shah, Ajay M; Wang, Qian; Zeng, Lingfang.

In: Stem Cells, Vol. 38, No. 4, 04.2020, p. 556-573.

Research output: Contribution to journalArticle

Harvard

Yang, J, Moraga, A, Xu, J, Zhao, Y, Luo, P, Lao, KH, Margariti, A, Zhao, Q, Ding, W, Wang, G, Zhang, M, Zheng, L, Zhang, Z, Hu, Y, Wang, W, Shen, L, Smith, A, Shah, AM, Wang, Q & Zeng, L 2020, 'A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation', Stem Cells, vol. 38, no. 4, pp. 556-573. https://doi.org/10.1002/stem.3122

APA

Yang, J., Moraga, A., Xu, J., Zhao, Y., Luo, P., Lao, K. H., ... Zeng, L. (2020). A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation. Stem Cells, 38(4), 556-573. https://doi.org/10.1002/stem.3122

Vancouver

Yang J, Moraga A, Xu J, Zhao Y, Luo P, Lao KH et al. A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation. Stem Cells. 2020 Apr;38(4):556-573. https://doi.org/10.1002/stem.3122

Author

Yang, Junyao ; Moraga, Ana ; Xu, Jing ; Zhao, Yue ; Luo, Peiyi ; Lao, Ka Hou ; Margariti, Andriana ; Zhao, Qiang ; Ding, Wei ; Wang, Gang ; Zhang, Min ; Zheng, Lei ; Zhang, Zhongyi ; Hu, Yanhua ; Wang, Wen ; Shen, Lisong ; Smith, Alberto ; Shah, Ajay M ; Wang, Qian ; Zeng, Lingfang. / A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation. In: Stem Cells. 2020 ; Vol. 38, No. 4. pp. 556-573.

Bibtex Download

@article{e4e3bf164fda4484a6236b35cb8f30d8,
title = "A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation",
abstract = "Histone deacetylase 7 (HDAC7) plays a pivotal role in the maintenance of the endothelium integrity. In this study, we demonstrated that the intron-containing Hdac7 mRNA existed in the cytosol and that ribosomes bound to a short open reading frame (sORF) within the 5′ terminal noncoding area of this Hdac7 mRNA in response to vascular endothelial growth factor (VEGF) stimulation in the isolated stem cell antigen-1 positive (Sca1 +) vascular progenitor cells (VPCs). A 7-amino acid (7A) peptide has been demonstrated to be translated from the sORF in Sca1 +-VPCs in vitro and in vivo. The 7A peptide was shown to receive phosphate group from the activated mitogen-activated protein kinase MEKK1 and transfer it to 14-3-3 gamma protein, forming an MEKK1-7A-14-3-3γ signal pathway downstream VEGF. The exogenous synthetic 7A peptide could increase Sca1 +-VPCs cell migration, reendothelialization in the femoral artery injury, and angiogenesis in hind limb ischemia. A Hd7-7sFLAG transgenic mice line was generated as the loss-of-function model, in which the 7A peptide was replaced by a FLAG-tagged scrabbled peptide. Loss of the endogenous 7A impaired Sca1 +-VPCs cell migration, reendothelialization of the injured femoral artery, and angiogenesis in ischemic tissues, which could be partially rescued by the addition of the exogenous 7A/7Ap peptide. This study provides evidence that sORFs can be alternatively translated and the derived peptides may play an important role in physiological processes including vascular remodeling.",
keywords = "histone deacetylase 7, peptide, phosphorylation, short open reading frame, vascular progenitor cell",
author = "Junyao Yang and Ana Moraga and Jing Xu and Yue Zhao and Peiyi Luo and Lao, {Ka Hou} and Andriana Margariti and Qiang Zhao and Wei Ding and Gang Wang and Min Zhang and Lei Zheng and Zhongyi Zhang and Yanhua Hu and Wen Wang and Lisong Shen and Alberto Smith and Shah, {Ajay M} and Qian Wang and Lingfang Zeng",
year = "2020",
month = "4",
doi = "10.1002/stem.3122",
language = "English",
volume = "38",
pages = "556--573",
journal = "Stem Cells",
issn = "1066-5099",
number = "4",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation

AU - Yang, Junyao

AU - Moraga, Ana

AU - Xu, Jing

AU - Zhao, Yue

AU - Luo, Peiyi

AU - Lao, Ka Hou

AU - Margariti, Andriana

AU - Zhao, Qiang

AU - Ding, Wei

AU - Wang, Gang

AU - Zhang, Min

AU - Zheng, Lei

AU - Zhang, Zhongyi

AU - Hu, Yanhua

AU - Wang, Wen

AU - Shen, Lisong

AU - Smith, Alberto

AU - Shah, Ajay M

AU - Wang, Qian

AU - Zeng, Lingfang

PY - 2020/4

Y1 - 2020/4

N2 - Histone deacetylase 7 (HDAC7) plays a pivotal role in the maintenance of the endothelium integrity. In this study, we demonstrated that the intron-containing Hdac7 mRNA existed in the cytosol and that ribosomes bound to a short open reading frame (sORF) within the 5′ terminal noncoding area of this Hdac7 mRNA in response to vascular endothelial growth factor (VEGF) stimulation in the isolated stem cell antigen-1 positive (Sca1 +) vascular progenitor cells (VPCs). A 7-amino acid (7A) peptide has been demonstrated to be translated from the sORF in Sca1 +-VPCs in vitro and in vivo. The 7A peptide was shown to receive phosphate group from the activated mitogen-activated protein kinase MEKK1 and transfer it to 14-3-3 gamma protein, forming an MEKK1-7A-14-3-3γ signal pathway downstream VEGF. The exogenous synthetic 7A peptide could increase Sca1 +-VPCs cell migration, reendothelialization in the femoral artery injury, and angiogenesis in hind limb ischemia. A Hd7-7sFLAG transgenic mice line was generated as the loss-of-function model, in which the 7A peptide was replaced by a FLAG-tagged scrabbled peptide. Loss of the endogenous 7A impaired Sca1 +-VPCs cell migration, reendothelialization of the injured femoral artery, and angiogenesis in ischemic tissues, which could be partially rescued by the addition of the exogenous 7A/7Ap peptide. This study provides evidence that sORFs can be alternatively translated and the derived peptides may play an important role in physiological processes including vascular remodeling.

AB - Histone deacetylase 7 (HDAC7) plays a pivotal role in the maintenance of the endothelium integrity. In this study, we demonstrated that the intron-containing Hdac7 mRNA existed in the cytosol and that ribosomes bound to a short open reading frame (sORF) within the 5′ terminal noncoding area of this Hdac7 mRNA in response to vascular endothelial growth factor (VEGF) stimulation in the isolated stem cell antigen-1 positive (Sca1 +) vascular progenitor cells (VPCs). A 7-amino acid (7A) peptide has been demonstrated to be translated from the sORF in Sca1 +-VPCs in vitro and in vivo. The 7A peptide was shown to receive phosphate group from the activated mitogen-activated protein kinase MEKK1 and transfer it to 14-3-3 gamma protein, forming an MEKK1-7A-14-3-3γ signal pathway downstream VEGF. The exogenous synthetic 7A peptide could increase Sca1 +-VPCs cell migration, reendothelialization in the femoral artery injury, and angiogenesis in hind limb ischemia. A Hd7-7sFLAG transgenic mice line was generated as the loss-of-function model, in which the 7A peptide was replaced by a FLAG-tagged scrabbled peptide. Loss of the endogenous 7A impaired Sca1 +-VPCs cell migration, reendothelialization of the injured femoral artery, and angiogenesis in ischemic tissues, which could be partially rescued by the addition of the exogenous 7A/7Ap peptide. This study provides evidence that sORFs can be alternatively translated and the derived peptides may play an important role in physiological processes including vascular remodeling.

KW - histone deacetylase 7

KW - peptide

KW - phosphorylation

KW - short open reading frame

KW - vascular progenitor cell

UR - http://www.scopus.com/inward/record.url?scp=85078298917&partnerID=8YFLogxK

U2 - 10.1002/stem.3122

DO - 10.1002/stem.3122

M3 - Article

VL - 38

SP - 556

EP - 573

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 4

ER -

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