King's College London

Funding Information: We would like to thank Mafalda Sousa (I3S, Porto), Ignacio Arganda‐Carreras (Universidad del Pais Vasco, Donostia‐San Sebastian), Martin Jones (The Francis Crick Institute, London) for reviewing the manuscript and providing constructive feedback prior to submission. We also would like to thank Michael Nelson (UW Madison), Sebastian Rhode (Zeiss), Ralph Palmisano (OICE Erlangen), Johannes Hohlbein (Wageningen UR), Kirti Prakash (ICR London) and Luciano Guerreiro Lucas (Leica Microsystems) for their constructive feedback about the preprint which we incorporated. RH acknowledges support by the Deutsche Forschungsgemeinschaft under Germany's Excellence Strategy – EXC2068 – Cluster of Excellence Physics of Life of TU Dresden. EF, IB and EJ acknowledge support by Biomedicum Imaging Unit and Electron Microscopy Unit, Helsinki University, as a part of Biocenter Finland infrastructure. CT acknowledges support by grant number 2020‐225265 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation. SC acknowledges support from a Royal Society University Research Fellowship. AK was funded via the BioImage Informatics Facility, a unit of the National Bioinformatics Infrastructure Sweden NBIS, with funding from SciLifeLab, National Microscopy Infrastructure NMI (VR‐RFI 2019‐00217), and the Chan‐Zuckerberg Initiative. Open Access funding enabled and organized by Projekt DEAL. Funding Information: We would like to thank Mafalda Sousa (I3S, Porto), Ignacio Arganda-Carreras (Universidad del Pais Vasco, Donostia-San Sebastian), Martin Jones (The Francis Crick Institute, London) for reviewing the manuscript and providing constructive feedback prior to submission. We also would like to thank Michael Nelson (UW Madison), Sebastian Rhode (Zeiss), Ralph Palmisano (OICE Erlangen), Johannes Hohlbein (Wageningen UR), Kirti Prakash (ICR London) and Luciano Guerreiro Lucas (Leica Microsystems) for their constructive feedback about the preprint which we incorporated. RH acknowledges support by the Deutsche Forschungsgemeinschaft under Germany's Excellence Strategy – EXC2068 – Cluster of Excellence Physics of Life of TU Dresden. EF, IB and EJ acknowledge support by Biomedicum Imaging Unit and Electron Microscopy Unit, Helsinki University, as a part of Biocenter Finland infrastructure. CT acknowledges support by grant number 2020-225265 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation. SC acknowledges support from a Royal Society University Research Fellowship. AK was funded via the BioImage Informatics Facility, a unit of the National Bioinformatics Infrastructure Sweden NBIS, with funding from SciLifeLab, National Microscopy Infrastructure NMI (VR-RFI 2019-00217), and the Chan-Zuckerberg Initiative. Open Access funding enabled and organized by Projekt DEAL. Publisher Copyright: © 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.