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A light-scattering assay for lymphocyte shape and its application to T and B lymphocyte responses to cultured high-walled endothelial cells

Research output: Contribution to journalArticle

H. Harris, C. Jolley, N.G. Miller

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalJournal of Immunological Methods
Volume192
Issue number1-2
DOIs
Published1996

Bibliographical note

Cited By :2 Export Date: 3 May 2016

King's Authors

Abstract

Lymphocyte shape changes are detectable as changes in the forward light scatter (FLS) profile of a Becton-Dickinson FACStar flow cytometer, with polar cells giving lower values of FLS. The FACScan cell analyser does not detect these changes. Freshly isolated lymph node lymphocytes are round, but when stimulated, for example by incubation with cultured high endothelial cells (HEC), a proportion change shape and become polar. The FLS profiles of lymphocytes stained for T and B cell surface markers show that both subsets change shape in response to HEC, but in a consistently different manner. Light microscopy of sorted T and B populations indicates that equal proportions of both cell types change shape, but that T cells are more likely to become highly elongated.

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