A lipophilic copper(II) complex as an optical probe for intracellular detection of NO

Neil Wilson; Lok Hang Mak; Agostino Cilibrizzi; Antony D. Gee; Nicholas J. Long; Rudiger Woscholski; Ramon Vilar

doi:10.1039/C6DT02880B

A lipophilic copper(II) complex as an optical probe for intracellular detection of NO

Neil Wilson, Lok Hang Mak, Agostino Cilibrizzi, Antony D. Gee, Nicholas J. Long, Rudiger Woscholski^*, Ramon Vilar

^*Corresponding author for this work

Imaging Chemistry & Biology

Imperial College London
Fédération d'anesthésiologie-réanimation et de médecine hyperbare, hôpital d'instruction des armées du Val-de-Grâce, 75005 Paris, France; Inserm U676, hôpital Robert-Debré, 48, boulevard Sérurier, 75019 Paris, France; Université Paris Diderot, Sorbonne Paris Cité, UMRS 676, 75013 Paris, France; Centre for the Developing Brain, Department of Perinatal Imaging and Health, King's College London, London, Royaume-Uni. Electronic address: [email protected].

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A new chemical sensor for cellular imaging of NO is presented. This cell-permeable probe is based on a complex where copper(ii) is coordinated to a tridentate ligand substituted with a fluorophore (NBD) and an octyl group. The fluorescence response of this complex towards a range of reactive species (namely NO, NO₂ ⁻, NO₃ ⁻, H₂O₂, ClO⁻, O₂ ⁻ and ONOO⁻) has been studied in vitro showing that the probe is highly selective for NO. The probe is readily taken up by cells and is able to image the cellular concentrations of NO.

Original language	English
Pages (from-to)	18177-18182
Number of pages	6
Journal	Dalton transactions (Cambridge, England : 2003)
Volume	45
Issue number	45
Early online date	18 Oct 2016
DOIs	https://doi.org/10.1039/C6DT02880B
Publication status	E-pub ahead of print - 18 Oct 2016

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abstract = "A new chemical sensor for cellular imaging of NO is presented. This cell-permeable probe is based on a complex where copper(ii) is coordinated to a tridentate ligand substituted with a fluorophore (NBD) and an octyl group. The fluorescence response of this complex towards a range of reactive species (namely NO, NO2 −, NO3 −, H2O2, ClO−, O2 − and ONOO−) has been studied in vitro showing that the probe is highly selective for NO. The probe is readily taken up by cells and is able to image the cellular concentrations of NO.",

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AU - Long, Nicholas J.

AU - Woscholski, Rudiger

AU - Vilar, Ramon

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A lipophilic copper(II) complex as an optical probe for intracellular detection of NO

Abstract

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