A mass spectrometry-based hybrid method for structural modeling of protein complexes

Anargyros Politis, Florian Stengel, Zoe Hall, Helena Hernández, Alexander Leitner, Thomas Walzthoeni, Carol V. Robinson, Ruedi Aebersold*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

134 Citations (Scopus)

Abstract

We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalNATURE METHODS
Volume11
Issue number4
Early online date9 Feb 2014
DOIs
Publication statusPublished - Apr 2014

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