A mass spectrometry-based hybrid method for structural modeling of protein complexes

TY - JOUR

T1 - A mass spectrometry-based hybrid method for structural modeling of protein complexes

AU - Politis, Anargyros

AU - Stengel, Florian

AU - Hall, Zoe

AU - Hernández, Helena

AU - Leitner, Alexander

AU - Walzthoeni, Thomas

AU - Robinson, Carol V.

AU - Aebersold, Ruedi

PY - 2014/4

Y1 - 2014/4

N2 - We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

AB - We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

UR - http://www.scopus.com/inward/record.url?scp=84897954111&partnerID=8YFLogxK

U2 - 10.1038/nmeth.2841

DO - 10.1038/nmeth.2841

M3 - Article

C2 - 24509631

AN - SCOPUS:84897954111

SN - 1548-7091

VL - 11

SP - 403

EP - 406

JO - NATURE METHODS

JF - NATURE METHODS

IS - 4

ER -