A Multifaceted Imbalance of T Cells with Regulatory Function Characterizes Type 1 Autoimmune Hepatitis

Silvia Ferri, Maria Serena Longhi, Chiara De Molo, Claudine Lalanne, Paolo Muratori, Alessandro Granito, Munther J. Hussain, Yun Ma, Marco Lenzi, Giorgina Mieli-Vergani, Francesco B. Bianchi, Diego Vergani, Luigi Muratori

    Research output: Contribution to journalArticlepeer-review

    163 Citations (Scopus)


    Immunotolerance is maintained by regulatory T cells (Tregs), including CD4(+)CD25(hi), CD8(+)CD28(-), gamma delta, and CD3(+)CD56(+) [natural killer T (NKT)] cells. CD4(+)CD25(hi) cells are impaired in children with autoimmune hepatitis (AM). Little is known about Tregs in adults with AIH. The aim of this study was to investigate the frequency and function of Treg subsets in adult patients with AIH during periods of active disease and remission. Forty-seven AIH patients (16 with active disease and 31 in remission) and 28 healthy controls were studied. Flow cytometry was used to evaluate surface markers and function-related intracellular molecules in gamma delta, CD8(+)CD28(-), NKT, and CD4(+)CD25(hi) cells. CD4(+)CD25(hi) T cell function was determined by the ability to suppress proliferation and interferon gamma (IFN-gamma) production by CD4(+)CD25(-) target cells. Liver forkhead box P3-positive (FOXP3(+)) cells were sought by immunohistochemistry. In AIH patients, particularly during active disease, CD4(+)CD25(hi) T cells were fewer, expressed lower levels of FOXP3, and were less effective at inhibiting target cell proliferation versus healthy controls. Moreover, although the numbers of CD8(+)CD28(-) T cells were similar in AIH patients and healthy controls, NKT cells were numerically reduced, especially during active disease, and produced lower quantities of the immunoregulatory cytokine interleukin-4 versus controls. In contrast, gamma delta T cells in AIH patients were more numerous versus healthy controls and had an inverted V delta 1/V delta 2 ratio and higher IFN-gamma and granzyme B production; the latter was correlated to biochemical indices of liver damage. There were few FOXP3(+) cells within the portal tract inflammatory infiltrate. Conclusion: Our data show that the defect in immunoregulation in adult AIH is complex, and gamma delta T cells are likely to be effectors of liver damage. (HEPATOLOGY 2010;52:999-1007)
    Original languageEnglish
    Pages (from-to)999 - 1007
    Number of pages9
    Issue number3
    Publication statusPublished - Sept 2010


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