A new function of ROD1 in nonsense-mediated mRNA decay

T F Brazão, J Demmers, W van IJcken, J Strouboulis, M Fornerod, L Romão, F G Grosveld

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

RNA-binding proteins play a crucial role in the post-transcriptional regulation of gene expression. Polypyrimidine tract binding protein (PTB in humans) has been extensively characterized as an important splicing factor, and has additional functions in 3' end processing and translation. ROD1 is a PTB paralog containing four RRM (RNA recognition motif) domains. Here, we discover a function of ROD1 in nonsense-mediated mRNA decay (NMD). We show that ROD1 and the core NMD factor UPF1 interact and co-regulate an extensive number of target genes. Using a reporter system, we demonstrate that ROD1, similarly to UPF1 and UPF2, is required for the destabilization of a known NMD substrate. Finally, we show through RIP-seq that ROD1 and UPF1 associate with a significant number of common transcripts.

Original languageEnglish
Pages (from-to)1101-10
Number of pages10
JournalFEBS Letters
Volume586
Issue number8
DOIs
Publication statusPublished - 24 Apr 2012

Keywords

  • Codon, Nonsense
  • HEK293 Cells
  • Humans
  • Nonsense Mediated mRNA Decay
  • Polypyrimidine Tract-Binding Protein/genetics
  • RNA Stability
  • RNA, Messenger/metabolism

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