TY - JOUR
T1 - A new model for the continuous monitoring of polymorphonuclear leukocyte trapping in the pulmonary vasculature of the rabbit
AU - Jones, H
AU - Paul, W
AU - Page, C P
PY - 2002/7
Y1 - 2002/7
N2 - Introduction: Accumulation of polymorphornuclear leukocytes (PMNs) within the pulmonary vasculature contributes to the pathophysiology of a number of diseases, including chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome. The techniques available to study this have their limitations. Methods: We have developed a minimally invasive technique for the continuous monitoring of In-111-labelled PMNs (In-111-PNMs) in the thoracic and groin regions of rabbits. The effects of intravenous injection of the chemoattractants, interleukin (IL)-8, leukotriene (LT) B-4, and lipopolysaccharide (LPS) were studied. Results: Intravenous injection of LTB4, IL-8, or LPS caused an accumulation of In-111-PMNs in the lung and a concomitant decrease in counts in the leg and caused no significant change from baseline in counts in the lung or the leg after injection of In-111-labelled red blood cells (RBCs). Conclusion: In conclusion, we have demonstrated a technique that is simple, reproducible, and robust, which can be used to investigate PMN trapping in the lung vasculature in response to well characterised PMN chemoattractants and that may be applied to the preclinical study of new drugs. (C) 2003 Elsevier Science Inc. All rights reserved.
AB - Introduction: Accumulation of polymorphornuclear leukocytes (PMNs) within the pulmonary vasculature contributes to the pathophysiology of a number of diseases, including chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome. The techniques available to study this have their limitations. Methods: We have developed a minimally invasive technique for the continuous monitoring of In-111-labelled PMNs (In-111-PNMs) in the thoracic and groin regions of rabbits. The effects of intravenous injection of the chemoattractants, interleukin (IL)-8, leukotriene (LT) B-4, and lipopolysaccharide (LPS) were studied. Results: Intravenous injection of LTB4, IL-8, or LPS caused an accumulation of In-111-PMNs in the lung and a concomitant decrease in counts in the leg and caused no significant change from baseline in counts in the lung or the leg after injection of In-111-labelled red blood cells (RBCs). Conclusion: In conclusion, we have demonstrated a technique that is simple, reproducible, and robust, which can be used to investigate PMN trapping in the lung vasculature in response to well characterised PMN chemoattractants and that may be applied to the preclinical study of new drugs. (C) 2003 Elsevier Science Inc. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=0038731373&partnerID=8YFLogxK
U2 - 10.1016/S1056-8719(03)00005-4
DO - 10.1016/S1056-8719(03)00005-4
M3 - Article
VL - 48
SP - 21
EP - 29
JO - Journal of Pharmacological and Toxicological Methods
JF - Journal of Pharmacological and Toxicological Methods
IS - 1
ER -