A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions

Katja Gehmlich, Pier D. Lambiase, Angeliki Asimaki, Edward J. Ciaccio, Elisabeth Ehler, Petros Syrris, Jeffrey E. Saffitz, William J. McKenna

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

BACKGROUND Cellular adhesion mediated by cardiac desmosomes is a prerequisite for proper electric propagation mediated by gap junctions in the myocardium. However, the molecular principles underlying this interdependence are not fully understood. OBJECTIVE The purpose of this study was to determine potential causes of right ventricular conduction abnormalities in a patient with borderline diagnosis of arrhythmogenic right ventricular cardiomyopathy. METHODS To assess molecular changes, the patient's myocardial tissue was analyzed for altered desmosomal and gap junction (connexin43) protein levels and localization. In vitro functional studies were performed to characterize the consequences of the desmosomal mutations. RESULTS Loss of plakoglobin signal was evident at the cell junctions despite expression of the protein at control levels. Although the distribution of connexin43 was not altered, total protein levels were reduced and changes in phosphorylation were observed. The truncation mutant in desmocollin-2a is deficient in binding plakoglobin. Moreover, the ability of desmocollin-2a to directly interact with connexin43 was abolished by the mutation. No pathogenic potential of the desmoglein-2 missense change was identified. CONCLUSION The observed abnormalities in gap junction protein expression and phosphorylation, which precede an overt cardiac phenotype, likely are responsible for slow myocardial conduction in this patient. At the molecular level, altered binding properties of the desmocollin-2a mutant may contribute to the changes in connexin43. In particular, the newly identified interaction between the desmocollin-2a isoform and connexin43 provides novel insights into the molecular link between desmosomes and gap junctions.
Original languageEnglish
Pages (from-to)711 - 718
Number of pages8
JournalHEART RHYTHM
Volume8
Issue number5
DOIs
Publication statusPublished - May 2011

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