A novel ex vivo culture system for studying bone repair

G. N. Gurav; G. Bailey; R. Sambrook; E. Tsiridis; Lucy Di-Silvio

doi:10.1016/j.injury.2006.08.019

A novel ex vivo culture system for studying bone repair

G. N. Gurav, G. Bailey, R. Sambrook, E. Tsiridis^*, Lucy Di-Silvio

^*Corresponding author for this work

Centre for Oral, Clinical & Translational Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Repair of large bony defects still remains a challenge for surgeons. Hydroxyapatite (HA) is well known for its biocompatibility and osseoconduction properties in the osseous environment. In this study the biofunctionality of a newly developed scaffold comprising of collagen and HA, with variable macropores was examined. The biological response was evaluated using primary human osteoblast cells (HOBs). Cell infiltration, proliferation and differentiation were assessed. The results showed that HOBs were able to migrate from the collagen into the HA pores with greater cell migration and infiltration observed in those scaffolds with larger pores. Furthermore, it was shown that Alkaline Phosphatase, a differentiation marker for HOBs was enhanced as the average macropore size increased. This in vitro model provides a more relevant method of testing the biofunctionality and migration ability of cells at a trauma site following implantation in bone and cartilage.

Original language	English
Pages (from-to)	S10-S17
Number of pages	8
Journal	Injury
Volume	37
Issue number	3 Supplement
DOIs	https://doi.org/10.1016/j.injury.2006.08.019
Publication status	Published - Mar 2007

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title = "A novel ex vivo culture system for studying bone repair",

abstract = "Repair of large bony defects still remains a challenge for surgeons. Hydroxyapatite (HA) is well known for its biocompatibility and osseoconduction properties in the osseous environment. In this study the biofunctionality of a newly developed scaffold comprising of collagen and HA, with variable macropores was examined. The biological response was evaluated using primary human osteoblast cells (HOBs). Cell infiltration, proliferation and differentiation were assessed. The results showed that HOBs were able to migrate from the collagen into the HA pores with greater cell migration and infiltration observed in those scaffolds with larger pores. Furthermore, it was shown that Alkaline Phosphatase, a differentiation marker for HOBs was enhanced as the average macropore size increased. This in vitro model provides a more relevant method of testing the biofunctionality and migration ability of cells at a trauma site following implantation in bone and cartilage.",

author = "Gurav, {G. N.} and G. Bailey and R. Sambrook and E. Tsiridis and Lucy Di-Silvio",

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TY - JOUR

T1 - A novel ex vivo culture system for studying bone repair

AU - Gurav, G. N.

AU - Bailey, G.

AU - Sambrook, R.

AU - Tsiridis, E.

AU - Di-Silvio, Lucy

PY - 2007/3

Y1 - 2007/3

N2 - Repair of large bony defects still remains a challenge for surgeons. Hydroxyapatite (HA) is well known for its biocompatibility and osseoconduction properties in the osseous environment. In this study the biofunctionality of a newly developed scaffold comprising of collagen and HA, with variable macropores was examined. The biological response was evaluated using primary human osteoblast cells (HOBs). Cell infiltration, proliferation and differentiation were assessed. The results showed that HOBs were able to migrate from the collagen into the HA pores with greater cell migration and infiltration observed in those scaffolds with larger pores. Furthermore, it was shown that Alkaline Phosphatase, a differentiation marker for HOBs was enhanced as the average macropore size increased. This in vitro model provides a more relevant method of testing the biofunctionality and migration ability of cells at a trauma site following implantation in bone and cartilage.

AB - Repair of large bony defects still remains a challenge for surgeons. Hydroxyapatite (HA) is well known for its biocompatibility and osseoconduction properties in the osseous environment. In this study the biofunctionality of a newly developed scaffold comprising of collagen and HA, with variable macropores was examined. The biological response was evaluated using primary human osteoblast cells (HOBs). Cell infiltration, proliferation and differentiation were assessed. The results showed that HOBs were able to migrate from the collagen into the HA pores with greater cell migration and infiltration observed in those scaffolds with larger pores. Furthermore, it was shown that Alkaline Phosphatase, a differentiation marker for HOBs was enhanced as the average macropore size increased. This in vitro model provides a more relevant method of testing the biofunctionality and migration ability of cells at a trauma site following implantation in bone and cartilage.

U2 - 10.1016/j.injury.2006.08.019

DO - 10.1016/j.injury.2006.08.019

M3 - Article

SN - 0020-1383

VL - 37

SP - S10-S17

JO - Injury

JF - Injury

IS - 3 Supplement

ER -

A novel ex vivo culture system for studying bone repair

Abstract

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