@article{76fcfe94fdce420a983b4fc3e9d87107,
title = "A novel immunoassay for malondialdehyde-conjugated low-density lipoprotein measures dynamic changes in the blood of patients undergoing coronary artery bypass graft surgery",
abstract = "Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r = 0.74, p < 0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable translational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability.",
keywords = "Anti-oxidized LDL antibodies, Antibodies, Assay, Atherosclerosis, Inflammation, Low-density lipoprotein (LDL), oxidized LDL, Malondialdehyde",
author = "Pandey, {Samata S.} and Adam Hartley and Mikhail Caga-Anan and Tareq Ammari and Khan, {Ameer Hamid Ahmed} and Nguyen, {Bao Anh Vu} and Chiari Kojima and Jon Anderson and Steven Lynham and Michael Johns and Haskard, {Dorian O.} and Khamis, {Ramzi Y.}",
note = "Funding Information: Funding: RK is funded by a BHF Clinical Research Fellowship and had received a Wellcome Trust Clinical Research Fellowship (095034/Z/10/Z) (as part of the Wellcome Trust/GSK Fellowship programme). DH received professorial chair funding from the British Heart Foundation (BHF). AH is funded by a Wellcome Trust Clinical Research Fellowship (220572/Z/20/Z). Funding from the National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) at Imperial College London was used for blood sample analysis. The Raissa and Anton Sansour Fund at Imperial Healthcare Charity also supported the research. Funding Information: RK is funded by a BHF Clinical Research Fellowship and had received a Wellcome Trust Clinical Research Fellowship (095034/Z/10/Z) (as part of the Wellcome Trust/GSK Fellowship pro-gramme). DH received professorial chair funding from the British Heart Foundation (BHF). AH is funded by a Wellcome Trust Clinical Research Fellowship (220572/Z/20/Z). Funding from the National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) at Imperial College London was used for blood sample analysis. The Raissa and Anton Sansour Fund at Imperial Healthcare Charity also supported the research. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = aug,
doi = "10.3390/antiox10081298",
language = "English",
volume = "10",
journal = "Antioxidants",
issn = "2076-3921",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "8",
}