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A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond

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A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells : Applications in trogocytosis and beyond. / Brown, Kathryn; Meader, Lucy; Nowocin, Anna; Edwards, Lindsey A.; Cheung, Lawrence H.; Smith, Richard A.; Rosenblum, Michael G.; Wong, Wilson.

In: Journal of Immunological Methods, Vol. 483, 112794, 08.2020, p. 1-10.

Research output: Contribution to journalArticle

Harvard

Brown, K, Meader, L, Nowocin, A, Edwards, LA, Cheung, LH, Smith, RA, Rosenblum, MG & Wong, W 2020, 'A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond', Journal of Immunological Methods, vol. 483, 112794, pp. 1-10. https://doi.org/10.1016/j.jim.2020.112794

APA

Brown, K., Meader, L., Nowocin, A., Edwards, L. A., Cheung, L. H., Smith, R. A., Rosenblum, M. G., & Wong, W. (2020). A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond. Journal of Immunological Methods, 483, 1-10. [112794]. https://doi.org/10.1016/j.jim.2020.112794

Vancouver

Brown K, Meader L, Nowocin A, Edwards LA, Cheung LH, Smith RA et al. A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond. Journal of Immunological Methods. 2020 Aug;483:1-10. 112794. https://doi.org/10.1016/j.jim.2020.112794

Author

Brown, Kathryn ; Meader, Lucy ; Nowocin, Anna ; Edwards, Lindsey A. ; Cheung, Lawrence H. ; Smith, Richard A. ; Rosenblum, Michael G. ; Wong, Wilson. / A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells : Applications in trogocytosis and beyond. In: Journal of Immunological Methods. 2020 ; Vol. 483. pp. 1-10.

Bibtex Download

@article{6269e585cef645f68fce728bc9f3fce5,
title = "A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond",
abstract = "A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of efficiency. Here, we describe an in vivo model combining the use of immunotoxins and multidrug resistant (MDR) gene deficient mice so that only MDR deficient cells expressing the target molecule would be depleted while target molecule expressing, but MDR sufficient, cells are spared. This allows targeted depletion at a higher degree of specificity than has been previously achieved. We have applied this technique to study trogocytosis, the intercellular transfer of cell surface molecules, but this principle could also be adapted using technology already available for use in other fields of study.",
keywords = "Antigen presentation, Cell depletion, Immunotoxin, Molecular transfer, Trogocytosis",
author = "Kathryn Brown and Lucy Meader and Anna Nowocin and Edwards, {Lindsey A.} and Cheung, {Lawrence H.} and Smith, {Richard A.} and Rosenblum, {Michael G.} and Wilson Wong",
year = "2020",
month = aug,
doi = "10.1016/j.jim.2020.112794",
language = "English",
volume = "483",
pages = "1--10",
journal = "Journal of Immunological Methods",
issn = "0022-1759",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells

T2 - Applications in trogocytosis and beyond

AU - Brown, Kathryn

AU - Meader, Lucy

AU - Nowocin, Anna

AU - Edwards, Lindsey A.

AU - Cheung, Lawrence H.

AU - Smith, Richard A.

AU - Rosenblum, Michael G.

AU - Wong, Wilson

PY - 2020/8

Y1 - 2020/8

N2 - A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of efficiency. Here, we describe an in vivo model combining the use of immunotoxins and multidrug resistant (MDR) gene deficient mice so that only MDR deficient cells expressing the target molecule would be depleted while target molecule expressing, but MDR sufficient, cells are spared. This allows targeted depletion at a higher degree of specificity than has been previously achieved. We have applied this technique to study trogocytosis, the intercellular transfer of cell surface molecules, but this principle could also be adapted using technology already available for use in other fields of study.

AB - A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of efficiency. Here, we describe an in vivo model combining the use of immunotoxins and multidrug resistant (MDR) gene deficient mice so that only MDR deficient cells expressing the target molecule would be depleted while target molecule expressing, but MDR sufficient, cells are spared. This allows targeted depletion at a higher degree of specificity than has been previously achieved. We have applied this technique to study trogocytosis, the intercellular transfer of cell surface molecules, but this principle could also be adapted using technology already available for use in other fields of study.

KW - Antigen presentation

KW - Cell depletion

KW - Immunotoxin

KW - Molecular transfer

KW - Trogocytosis

UR - http://www.scopus.com/inward/record.url?scp=85084991299&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2020.112794

DO - 10.1016/j.jim.2020.112794

M3 - Article

C2 - 32428450

AN - SCOPUS:85084991299

VL - 483

SP - 1

EP - 10

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

M1 - 112794

ER -

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