A novel locomotion-based prepulse inhibition assay in zebrafish larvae

Emily Read; Robert Hindges

doi:10.17912/micropub.biology.000914

A novel locomotion-based prepulse inhibition assay in zebrafish larvae

Emily Read
, Robert Hindges^*

^*Corresponding author for this work

Developmental Neurobiology

Research output: Contribution to journal › Article › peer-review

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Abstract

Sensory gating, measured using prepulse inhibition (PPI), is an endophenotype of neuropsychiatric disorders that can be assessed in larval zebrafish models. However, current PPI assays require high-speed cameras to capture rapid c-bend startle behaviours of the larvae. In this study, we designed and employed a PPI paradigm that uses locomotion as a read-out of zebrafish larval startle responses. PPI percentage was measured at a maximum of 87% and strongly reduced upon administration of the NMDA receptor antagonist, MK-801. This work provides the foundation for simpler and more accessible PPI assays using larval zebrafish to model key endophenotypes of neurodevelopmental disorders.

Original language	English
Number of pages	8
Journal	microPublication Biology
Volume	2024
DOIs	https://doi.org/10.17912/micropub.biology.000914
Publication status	Published - 24 Jan 2024

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A novel locomotion-based_READ_Publishedonline24January2024_GOLD_VoR (CC BY)Final published version, 924 KBLicence: CC BY

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abstract = "Sensory gating, measured using prepulse inhibition (PPI), is an endophenotype of neuropsychiatric disorders that can be assessed in larval zebrafish models. However, current PPI assays require high-speed cameras to capture rapid c-bend startle behaviours of the larvae. In this study, we designed and employed a PPI paradigm that uses locomotion as a read-out of zebrafish larval startle responses. PPI percentage was measured at a maximum of 87\% and strongly reduced upon administration of the NMDA receptor antagonist, MK-801. This work provides the foundation for simpler and more accessible PPI assays using larval zebrafish to model key endophenotypes of neurodevelopmental disorders.",

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A novel locomotion-based prepulse inhibition assay in zebrafish larvae

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