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A novel method to quantify dentine tubule occlusion applied to in-situ model samples

Research output: Contribution to journalArticle

Ryan Olley, Charles Parkinson, R Wilson, Rebecca Moazzez, David Bartlett

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalCaries research
Volume48
Issue number1
Early online date6 Nov 2013
DOIs
Publication statusPublished - 2014

King's Authors

Abstract

Objectives: To develop an innovative computerised routine, in conjunction with a minimally destructive imaging technique, to quantify dentine tubule occlusion.
Methods: Polished human dentine samples (n = 480) were brushed during a 4-day in situ study with two occlusion-based dentifrices, a sodium fluoride control dentifrice (1,450 ppm) or water. Samples were imaged with tandem scanning microscopy (TSM) and conventional scanning electron microscopy (SEM). The level of dentine patency was then assessed using a visual ordinal scale (‘standard') or quantitatively using a specially designed computational routine.
Results: The occlusion-based dentifrice resulted in significantly less patent dentine than controls for the ‘standard' (p = 0.01) assessment, but not for computer analyses (p = 0.10). The correlation of the number of individual patent tubules counted visually and by the computational routine in calibrations and in the in situ study was ≥0.8.
Conclusions: This study identified a new computer-based routine, capable of objectively quantifying the patency of dentine imaged by SEM and TSM.

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