A peripheral nervous system actin-binding protein regulates neurite outgrowth

TY - JOUR

T1 - A peripheral nervous system actin-binding protein regulates neurite outgrowth

AU - Ravenall, S J

AU - Gavazzi, I

AU - Wood, J N

AU - Akopian, A N

PY - 2002

Y1 - 2002

N2 - Difference cloning has identified a Villin-like mRNA transcript expressed selectively in peripheral sensory and sympathetic neurons. Pervin, the encoded 820-amino acid protein, has 60% identity with - Villin and is the rat homologue of Advillin. Coimmunoprecipitation studies demonstrate that Pervin and actin interact in vivo. Transfection of COS-7 epithelial cell lines demonstrates colocalization of epitope-tagged Pervin with green fluorescent protein-actin and results in an increase in process formation. This effect is abolished when the putative actin-bundling headpiece of Pervin is deleted. Biolistic transfection of primary cultures of rat dorsal root ganglion sensory neurons also results in increased neurite outgrowth with FLAG-tagged Pervin. Deletion of the actin-bundling headpiece inhibits normal neurite growth. These data suggest that Pervin may play a significant role in regulating process outgrowth in peripheral neurons through a mechanism that involves the activity of an actin-bundling domain.

AB - Difference cloning has identified a Villin-like mRNA transcript expressed selectively in peripheral sensory and sympathetic neurons. Pervin, the encoded 820-amino acid protein, has 60% identity with - Villin and is the rat homologue of Advillin. Coimmunoprecipitation studies demonstrate that Pervin and actin interact in vivo. Transfection of COS-7 epithelial cell lines demonstrates colocalization of epitope-tagged Pervin with green fluorescent protein-actin and results in an increase in process formation. This effect is abolished when the putative actin-bundling headpiece of Pervin is deleted. Biolistic transfection of primary cultures of rat dorsal root ganglion sensory neurons also results in increased neurite outgrowth with FLAG-tagged Pervin. Deletion of the actin-bundling headpiece inhibits normal neurite growth. These data suggest that Pervin may play a significant role in regulating process outgrowth in peripheral neurons through a mechanism that involves the activity of an actin-bundling domain.

UR - http://www.scopus.com/inward/record.url?scp=0036460145&partnerID=8YFLogxK

U2 - 10.1046/j.0953-816x.2001.01862.x

DO - 10.1046/j.0953-816x.2001.01862.x

M3 - Article

SN - 1460-9568

VL - 15

SP - 281

EP - 290

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

IS - 2

ER -