A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young finnish subjects

Elisabet Einarsdottir; Minna Pekkinen; Kaarel Krjutškov; Shintaro Katayama; Juha Kere; Outi Mäkitie; Heli Viljakainen

doi:10.1530/EC-18-0537

A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young finnish subjects

Elisabet Einarsdottir, Minna Pekkinen, Kaarel Krjutškov, Shintaro Katayama, Juha Kere, Outi Mäkitie, Heli Viljakainen^*

^*Corresponding author for this work

Medical & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

109 Downloads (Pure)

Abstract

Objective:

The effect of vitamin D at the transcriptome level is poorly understood, and furthermore, it is unclear if it differs between obese and normal-weight subjects. The objective of the study was to explore the transcriptome effects of vitamin D supplementation.

Design and methods:

We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (±s.d.) age 20.4 (±2.5) years and BMIs 36 (±10) and 23 (±4) kg/m ² , respectively. The supplemental daily vitamin D dose was 50 µg (2000 IU). Data were available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects.

Results:

Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted P value 3.08 × 10 ^-14 ). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects.

Conclusions:

Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level.

Original language	English
Pages (from-to)	462-467
Number of pages	6
Journal	Endocrine Connections
Volume	8
Issue number	5
DOIs	https://doi.org/10.1530/EC-18-0537
Publication status	Published - May 2019

Keywords

Gene expression
Intervention
Mitochondrial function
Obesity
Transcriptome
Vitamin D

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1530/EC-18-0537Licence: CC BY-NC-ND

preliminary transcriptome analysis_EINARSDOTTIR_Accepted9April2019_GOLD VoRFinal published version, 1.7 MBLicence: CC BY-NC-ND

Cite this

@article{1a0689945f394a76815843c5bf94d4a5,

title = "A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young finnish subjects",

abstract = " Objective: The effect of vitamin D at the transcriptome level is poorly understood, and furthermore, it is unclear if it differs between obese and normal-weight subjects. The objective of the study was to explore the transcriptome effects of vitamin D supplementation. Design and methods: We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (±s.d.) age 20.4 (±2.5) years and BMIs 36 (±10) and 23 (±4) kg/m 2 , respectively. The supplemental daily vitamin D dose was 50 µg (2000 IU). Data were available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects. Results: Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted P value 3.08 × 10 -14 ). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects. Conclusions: Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level. ",

keywords = "Gene expression, Intervention, Mitochondrial function, Obesity, Transcriptome, Vitamin D",

author = "Elisabet Einarsdottir and Minna Pekkinen and Kaarel Krjut{\v s}kov and Shintaro Katayama and Juha Kere and Outi M{\"a}kitie and Heli Viljakainen",

year = "2019",

month = may,

doi = "10.1530/EC-18-0537",

language = "English",

volume = "8",

pages = "462--467",

journal = "Endocrine Connections",

issn = "2049-3614",

publisher = "BioScientifica Ltd.",

number = "5",

}

TY - JOUR

T1 - A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young finnish subjects

AU - Einarsdottir, Elisabet

AU - Pekkinen, Minna

AU - Krjutškov, Kaarel

AU - Katayama, Shintaro

AU - Kere, Juha

AU - Mäkitie, Outi

AU - Viljakainen, Heli

PY - 2019/5

Y1 - 2019/5

N2 - Objective: The effect of vitamin D at the transcriptome level is poorly understood, and furthermore, it is unclear if it differs between obese and normal-weight subjects. The objective of the study was to explore the transcriptome effects of vitamin D supplementation. Design and methods: We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (±s.d.) age 20.4 (±2.5) years and BMIs 36 (±10) and 23 (±4) kg/m 2 , respectively. The supplemental daily vitamin D dose was 50 µg (2000 IU). Data were available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects. Results: Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted P value 3.08 × 10 -14 ). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects. Conclusions: Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level.

AB - Objective: The effect of vitamin D at the transcriptome level is poorly understood, and furthermore, it is unclear if it differs between obese and normal-weight subjects. The objective of the study was to explore the transcriptome effects of vitamin D supplementation. Design and methods: We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (±s.d.) age 20.4 (±2.5) years and BMIs 36 (±10) and 23 (±4) kg/m 2 , respectively. The supplemental daily vitamin D dose was 50 µg (2000 IU). Data were available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects. Results: Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted P value 3.08 × 10 -14 ). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects. Conclusions: Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level.

KW - Gene expression

KW - Intervention

KW - Mitochondrial function

KW - Obesity

KW - Transcriptome

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=85065989051&partnerID=8YFLogxK

U2 - 10.1530/EC-18-0537

DO - 10.1530/EC-18-0537

M3 - Article

AN - SCOPUS:85065989051

SN - 2049-3614

VL - 8

SP - 462

EP - 467

JO - Endocrine Connections

JF - Endocrine Connections

IS - 5

ER -

A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young finnish subjects

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this