King's College London

Research portal

A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations

Research output: Contribution to journalArticle

Standard

A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations. / Onesto, Valentina; Barrell, William B.; Okesola, Mary; Amato, Francesco; Gentile, Francesco; Liu, Karen J.; Chiappini, Ciro.

In: BIOMEDICAL MICRODEVICES, Vol. 21, No. 2, 44, 01.06.2019.

Research output: Contribution to journalArticle

Harvard

Onesto, V, Barrell, WB, Okesola, M, Amato, F, Gentile, F, Liu, KJ & Chiappini, C 2019, 'A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations', BIOMEDICAL MICRODEVICES, vol. 21, no. 2, 44. https://doi.org/10.1007/s10544-019-0390-0

APA

Onesto, V., Barrell, W. B., Okesola, M., Amato, F., Gentile, F., Liu, K. J., & Chiappini, C. (2019). A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations. BIOMEDICAL MICRODEVICES, 21(2), [44]. https://doi.org/10.1007/s10544-019-0390-0

Vancouver

Onesto V, Barrell WB, Okesola M, Amato F, Gentile F, Liu KJ et al. A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations. BIOMEDICAL MICRODEVICES. 2019 Jun 1;21(2). 44. https://doi.org/10.1007/s10544-019-0390-0

Author

Onesto, Valentina ; Barrell, William B. ; Okesola, Mary ; Amato, Francesco ; Gentile, Francesco ; Liu, Karen J. ; Chiappini, Ciro. / A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations. In: BIOMEDICAL MICRODEVICES. 2019 ; Vol. 21, No. 2.

Bibtex Download

@article{22e1c88e882d48c380d4a6de3c2649ea,
title = "A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations",
abstract = "In embryogenesis, mesenchymal condensation is a critical event during the formation of many organ systems, including cartilage and bone. During organ formation, mesenchymal cells aggregate and undergo compaction while activating developmental programmes. The final three-dimensional form of the organ, as well as cell fates, can be influenced by the size and shape of the forming condensation. This process is hypothesized to result from multiscale cell interactions within mesenchymal microenvironments; however, these are complex to investigate in vivo. Three-dimensional in vitro models that recapitulate key phenotypes can contribute to our understanding of the microenvironment interactions regulating this fundamental developmental process. Here we devise such models by using image analysis to guide the design of polydimethylsiloxane 3D microstructures as cell culture substrates. These microstructures establish geometrically constrained micromass cultures of mouse embryonic skeletal progenitor cells which influence the development of condensations. We first identify key phenotypes differentiating face and limb bud micromass cultures by linear discriminant analysis of the shape descriptors for condensation morphology, which are used to guide the rational design of a micropatterned polydimethylsiloxane substrate. High-content imaging analysis highlights that the geometry of the microenvironment affects the establishment and growth of condensations. Further, cells commit to establish condensations within the first 5 h; condensations reach their full size within 17 h; following which they increase cell density while maintaining size for at least 7 days. These findings elucidate the value of our model in dissecting key aspects of mesenchymal condensation development.",
author = "Valentina Onesto and Barrell, {William B.} and Mary Okesola and Francesco Amato and Francesco Gentile and Liu, {Karen J.} and Ciro Chiappini",
year = "2019",
month = "6",
day = "1",
doi = "10.1007/s10544-019-0390-0",
language = "English",
volume = "21",
journal = "BIOMEDICAL MICRODEVICES",
issn = "1387-2176",
publisher = "Kluwer Academic Publishers",
number = "2",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - A quantitative approach for determining the role of geometrical constraints when shaping mesenchymal condensations

AU - Onesto, Valentina

AU - Barrell, William B.

AU - Okesola, Mary

AU - Amato, Francesco

AU - Gentile, Francesco

AU - Liu, Karen J.

AU - Chiappini, Ciro

PY - 2019/6/1

Y1 - 2019/6/1

N2 - In embryogenesis, mesenchymal condensation is a critical event during the formation of many organ systems, including cartilage and bone. During organ formation, mesenchymal cells aggregate and undergo compaction while activating developmental programmes. The final three-dimensional form of the organ, as well as cell fates, can be influenced by the size and shape of the forming condensation. This process is hypothesized to result from multiscale cell interactions within mesenchymal microenvironments; however, these are complex to investigate in vivo. Three-dimensional in vitro models that recapitulate key phenotypes can contribute to our understanding of the microenvironment interactions regulating this fundamental developmental process. Here we devise such models by using image analysis to guide the design of polydimethylsiloxane 3D microstructures as cell culture substrates. These microstructures establish geometrically constrained micromass cultures of mouse embryonic skeletal progenitor cells which influence the development of condensations. We first identify key phenotypes differentiating face and limb bud micromass cultures by linear discriminant analysis of the shape descriptors for condensation morphology, which are used to guide the rational design of a micropatterned polydimethylsiloxane substrate. High-content imaging analysis highlights that the geometry of the microenvironment affects the establishment and growth of condensations. Further, cells commit to establish condensations within the first 5 h; condensations reach their full size within 17 h; following which they increase cell density while maintaining size for at least 7 days. These findings elucidate the value of our model in dissecting key aspects of mesenchymal condensation development.

AB - In embryogenesis, mesenchymal condensation is a critical event during the formation of many organ systems, including cartilage and bone. During organ formation, mesenchymal cells aggregate and undergo compaction while activating developmental programmes. The final three-dimensional form of the organ, as well as cell fates, can be influenced by the size and shape of the forming condensation. This process is hypothesized to result from multiscale cell interactions within mesenchymal microenvironments; however, these are complex to investigate in vivo. Three-dimensional in vitro models that recapitulate key phenotypes can contribute to our understanding of the microenvironment interactions regulating this fundamental developmental process. Here we devise such models by using image analysis to guide the design of polydimethylsiloxane 3D microstructures as cell culture substrates. These microstructures establish geometrically constrained micromass cultures of mouse embryonic skeletal progenitor cells which influence the development of condensations. We first identify key phenotypes differentiating face and limb bud micromass cultures by linear discriminant analysis of the shape descriptors for condensation morphology, which are used to guide the rational design of a micropatterned polydimethylsiloxane substrate. High-content imaging analysis highlights that the geometry of the microenvironment affects the establishment and growth of condensations. Further, cells commit to establish condensations within the first 5 h; condensations reach their full size within 17 h; following which they increase cell density while maintaining size for at least 7 days. These findings elucidate the value of our model in dissecting key aspects of mesenchymal condensation development.

U2 - 10.1007/s10544-019-0390-0

DO - 10.1007/s10544-019-0390-0

M3 - Article

VL - 21

JO - BIOMEDICAL MICRODEVICES

JF - BIOMEDICAL MICRODEVICES

SN - 1387-2176

IS - 2

M1 - 44

ER -

View graph of relations

© 2018 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454