A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

TY - JOUR

T1 - A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

AU - An, Zhengwen

AU - Sabalic, Maja

AU - Bloomquist, Ryan F.

AU - Fowler, Teresa E.

AU - Streelman, Todd

AU - Sharpe, Paul T.

PY - 2018/1

Y1 - 2018/1

N2 - The extent to which heterogeneity within mesenchymal stem cell (MSC) populations is related to function is not understood. Using the archetypal MSC in vitro surface marker, CD90/Thy1, here we show that 30% of the MSCs in the continuously growing mouse incisor express CD90/Thy1 and these cells give rise to 30% of the differentiated cell progeny during postnatal development. In adulthood, when growth rate homeostasis is established, the CD90/Thy1+ MSCs decrease dramatically in number. When adult incisors are cut, the growth rate increases to rapidly re-establish tooth length and homeostasis. This accelerated growth rate correlates with the re-appearance of CD90/Thy+ MSCs and re-establishment of their contribution to cell differentiation. A population of Celsr1+ quiescent cells becomes mitotic following clipping and replenishes the CD90/Thy1 population. A sub-population of MSCs thus exists in the mouse incisor, distinguished by expression of CD90/Thy1 that plays a specific role only during periods of increased growth rate.

AB - The extent to which heterogeneity within mesenchymal stem cell (MSC) populations is related to function is not understood. Using the archetypal MSC in vitro surface marker, CD90/Thy1, here we show that 30% of the MSCs in the continuously growing mouse incisor express CD90/Thy1 and these cells give rise to 30% of the differentiated cell progeny during postnatal development. In adulthood, when growth rate homeostasis is established, the CD90/Thy1+ MSCs decrease dramatically in number. When adult incisors are cut, the growth rate increases to rapidly re-establish tooth length and homeostasis. This accelerated growth rate correlates with the re-appearance of CD90/Thy+ MSCs and re-establishment of their contribution to cell differentiation. A population of Celsr1+ quiescent cells becomes mitotic following clipping and replenishes the CD90/Thy1 population. A sub-population of MSCs thus exists in the mouse incisor, distinguished by expression of CD90/Thy1 that plays a specific role only during periods of increased growth rate.

KW - Mesenchymal stem cells

U2 - 10.1038/s41467-017-02785-6

DO - 10.1038/s41467-017-02785-6

M3 - Article

SN - 2041-1723

VL - 9

JO - Nat Commun

JF - Nat Commun

IS - 378

M1 - 378

ER -