A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay

Phillip E. Morgan; Danielle Fisher; Richard Evers; Bob Flanagan

doi:10.1002/bmc.1515

A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay

Phillip E. Morgan, Danielle Fisher, Richard Evers, Bob Flanagan

King's College Hospital

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation-exchange (SCX)-modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC-UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra- and inter-assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples (n = 32). Analysis of patient samples (n = 115) using the HPLC method and the Seradyn QMS (R) Lamotrigine immunoassay showed that the immunoassay over-estimated lamotrigine by 21% on average.

Original language	English
Pages (from-to)	775 - 778
Number of pages	4
Journal	Biomedical Chromatography
Volume	25
Issue number	7
DOIs	https://doi.org/10.1002/bmc.1515
Publication status	Published - Jul 2011

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10.1002/bmc.1515

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title = "A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay",

abstract = "Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation-exchange (SCX)-modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC-UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra- and inter-assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples (n = 32). Analysis of patient samples (n = 115) using the HPLC method and the Seradyn QMS (R) Lamotrigine immunoassay showed that the immunoassay over-estimated lamotrigine by 21% on average.",

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T1 - A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay

AU - Morgan, Phillip E.

AU - Fisher, Danielle

AU - Evers, Richard

AU - Flanagan, Bob

PY - 2011/7

Y1 - 2011/7

N2 - Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation-exchange (SCX)-modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC-UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra- and inter-assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples (n = 32). Analysis of patient samples (n = 115) using the HPLC method and the Seradyn QMS (R) Lamotrigine immunoassay showed that the immunoassay over-estimated lamotrigine by 21% on average.

AB - Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation-exchange (SCX)-modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC-UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra- and inter-assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples (n = 32). Analysis of patient samples (n = 115) using the HPLC method and the Seradyn QMS (R) Lamotrigine immunoassay showed that the immunoassay over-estimated lamotrigine by 21% on average.

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DO - 10.1002/bmc.1515

M3 - Article

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JO - Biomedical Chromatography

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IS - 7

ER -

A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay

Abstract

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