A recombinant human HLA-class I antigen linked to dextran elicits innate and adaptive immune responses

Jorgen Scholler, Mahavir Singh, Lesley Bergmeier, Katja Brunstedt, Yufei Wang, Trevor Whittall, Durdana Rahman, J. Pido-Lopez, T. Lehner

Research output: Contribution to journalLiterature reviewpeer-review

16 Citations (Scopus)

Abstract

The objective of this study was to produce and evaluate the immunogenic potential of a recombinant HLA-class I antigen linked to dextran. The HLA-A*0201 heavy chain and 132 microglobulin were cloned by PCR amplification of overlapping oligonucleotides and produced in E. coli. These were assembled with a CMV binding peptide motif, the HLA complex was biotinylated and bound by streptavidin coated dextran at a ratio of 24 HLA to 1 dextran molecule (termed Dextramer). Allostimulation of human PBMC in vitro and in vivo immunization of Balb c mice with the HLA-A*0201 construct elicited CD4(+) and CD8(+) T cell proliferative responses, IgG specific antibodies in mice and in human T cell proliferation and APOBEC3G mRNA. These adaptive and innate immune responses induced by a novel recombinant HLA construct in human cells and mice suggest their application as a potential vaccine candidate against HIV infection. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)1 - 9
Number of pages9
JournalJournal of Immunological Methods
Volume360
Issue number1-2
DOIs
Publication statusPublished - Aug 2010

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