A review of analytical parameters in ‘rapid’ liquid chromatographic methods for bioanalysis: Can we do better?

Katie Lawlor, Erika Castrignano, Kim Wolff, Lewis Couchman, Anthony M. Edge, Atholl Johnston, Jake Clausen

Research output: Contribution to journalReview articlepeer-review

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Abstract

Rapid bioanalysis is beneficial to many applications. However, how ‘rapid’ a method is, or could be, is often an
unanswered question. In this statistical review, the authors have assessed multiple pre-analytical (i.e. sample
preparation), and analytical method parameters specifically for liquid chromatography to assist researchers in
developing and validating ‘rapid’ bioanalytical methods. We restricted the search to urine and plasma matrices
only. Data were extracted from over 2,000 recent studies and evaluated to assess how these parameters affected
the ‘on-instrument’ analysis time. In addition to methods using ultra-violet (UV) detection, there were a large
number of mass spectrometric (MS) methods, allowing additional review of the differences between high- and
low-resolution MS on analysis time. We observed that most (N = 922, 70 %) methods used 5 or 10 cm columns,
and that whilst uptake of ultra-high performance (U)HPLC columns was good, the use of sub-5 cm columns and/
or flow rates in excess of 1 mL/min was incredibly rare (N = 25, 3 %). The detector of choice for quantitative (U)
HPLC-MS remains the triple quadrupole, although a number of groups report the use of high-resolution MS for
such methods.
Original languageEnglish
Article number464803
JournalJournal of Chromatography A
Volume1721
DOIs
Publication statusPublished - 26 Apr 2024

Keywords

  • Bioanalysis
  • Liquid Chromatography
  • Mass Spectrometry
  • Plasma
  • Rapid
  • Urine

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