A rise of MMP-2 and MMP-9 in bronchoalveolar lavage fluid is associated with acute lung injury after cardiopulmonary bypass in a swine model

TY - JOUR

T1 - A rise of MMP-2 and MMP-9 in bronchoalveolar lavage fluid is associated with acute lung injury after cardiopulmonary bypass in a swine model

AU - Eichler, Wolfgang

AU - Bechtel, J F Matthias

AU - Schumacher, Jan

AU - Wermelt, Johanna A

AU - Klotz, Karl-Friedrich

AU - Bartels, Claus

PY - 2003/4

Y1 - 2003/4

N2 - Postoperative acute lung injury (ALI) contributes to the morbidity and mortality following cardiopulmonary bypass (CPB). To determine whether the presence of matrix metalloproteinases (MMPs) is associated with ALI after CPB, MMP-2 and MMP-9 activities in bronchoalveolar lavage fluid (BALF) were compared with parameters indicating impaired gas exchange. In a prospective study, 17 minipigs were subjected to CPB for 60 min. Before and at five and 180 min after CPB, MMP-2 and MMP-9 were assayed in BALF and the arterial-alveolar gradient of oxygen tension (AaDO2), the pulmonary capillary wedge pressure (PCWP) and the water content of lung tissue samples (Wt) were evaluated and compared with baseline values. MMP-2 and MMP-9 increased significantly 5 minutes (2.1- and 6.2-fold, respectively) and 180 minutes (3.4- and 14.3-fold, respectively) post-CPB. AaDO2 and Wt, but not PCWP, increased significantly 180 minutes after CPB and only AaDO2, but not PCWP or Wt, was significantly correlated with MMP-2 (r = 0.66, p = 0.006) and MMP-9 (r = 0.62, p = 0.01). In conclusion, high levels of MMP-2 and MMP-9 in the pulmonary compartment are associated with ALI after CPB.

AB - Postoperative acute lung injury (ALI) contributes to the morbidity and mortality following cardiopulmonary bypass (CPB). To determine whether the presence of matrix metalloproteinases (MMPs) is associated with ALI after CPB, MMP-2 and MMP-9 activities in bronchoalveolar lavage fluid (BALF) were compared with parameters indicating impaired gas exchange. In a prospective study, 17 minipigs were subjected to CPB for 60 min. Before and at five and 180 min after CPB, MMP-2 and MMP-9 were assayed in BALF and the arterial-alveolar gradient of oxygen tension (AaDO2), the pulmonary capillary wedge pressure (PCWP) and the water content of lung tissue samples (Wt) were evaluated and compared with baseline values. MMP-2 and MMP-9 increased significantly 5 minutes (2.1- and 6.2-fold, respectively) and 180 minutes (3.4- and 14.3-fold, respectively) post-CPB. AaDO2 and Wt, but not PCWP, increased significantly 180 minutes after CPB and only AaDO2, but not PCWP or Wt, was significantly correlated with MMP-2 (r = 0.66, p = 0.006) and MMP-9 (r = 0.62, p = 0.01). In conclusion, high levels of MMP-2 and MMP-9 in the pulmonary compartment are associated with ALI after CPB.

KW - Animals

KW - Bronchoalveolar Lavage Fluid

KW - Cardiopulmonary Bypass

KW - Lung

KW - Male

KW - Matrix Metalloproteinase 2

KW - Matrix Metalloproteinase 9

KW - Models, Animal

KW - Pulmonary Gas Exchange

KW - Pulmonary Wedge Pressure

KW - Respiratory Distress Syndrome, Adult

KW - Swine

KW - Water

KW - Journal Article

U2 - 10.1191/0267659103pf662oa

DO - 10.1191/0267659103pf662oa

M3 - Article

C2 - 12868788

SN - 0267-6591

VL - 18

SP - 107

EP - 113

JO - Perfusion

JF - Perfusion

IS - 2

ER -