A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis

Javier Barallobre-Barreiro; Athanasios Didangelos; Xiaoke Yin; Nieves Doménech; Manuel Mayr

doi:10.1007/978-1-62703-386-2_17

A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis

Javier Barallobre-Barreiro, Athanasios Didangelos, Xiaoke Yin, Nieves Doménech, Manuel Mayr

Cardiovascular Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

22 Citations (Scopus)

Abstract

Cardiacfibrosis is characterized by excessive deposition of extracellular matrix (ECM) and is a common complication of various cardiovascular diseases. However, little is known about proteins in the cardiac extracellular space. Proteomics analysis of cardiac ECM can be challenging due to the presence of more abundant intracellular proteins, the low degree of solubility of integral ECM proteins, and the presence of abundant posttranslational modifications. Here we describe an extraction methodology based on tissue decellularization, which allows the biochemical subfractionation of extracellular proteins in cardiac tissue. These relatively low-complexity protein fractions are suitable for analysis by gel-LC-MS/MS and other proteomics techniques.

Original language	English
Title of host publication	Heart Proteomics
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc
Pages	215-223
Number of pages	9
ISBN (Print)	9781627033855
DOIs	https://doi.org/10.1007/978-1-62703-386-2_17
Publication status	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	1005
ISSN (Print)	1064-3745

Keywords

Cardiacfibrosis
Extracellular matrix
Fractionation techniques

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-62703-386-2_17

Cite this

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abstract = "Cardiacfibrosis is characterized by excessive deposition of extracellular matrix (ECM) and is a common complication of various cardiovascular diseases. However, little is known about proteins in the cardiac extracellular space. Proteomics analysis of cardiac ECM can be challenging due to the presence of more abundant intracellular proteins, the low degree of solubility of integral ECM proteins, and the presence of abundant posttranslational modifications. Here we describe an extraction methodology based on tissue decellularization, which allows the biochemical subfractionation of extracellular proteins in cardiac tissue. These relatively low-complexity protein fractions are suitable for analysis by gel-LC-MS/MS and other proteomics techniques.",

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A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis. / Barallobre-Barreiro, Javier ; Didangelos, Athanasios ; Yin, Xiaoke et al.
Heart Proteomics: Methods and Protocols. Humana Press Inc, 2013. p. 215-223 (Methods in Molecular Biology; Vol. 1005).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis

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AU - Didangelos, Athanasios

AU - Yin, Xiaoke

AU - Doménech, Nieves

AU - Mayr, Manuel

PY - 2013

Y1 - 2013

N2 - Cardiacfibrosis is characterized by excessive deposition of extracellular matrix (ECM) and is a common complication of various cardiovascular diseases. However, little is known about proteins in the cardiac extracellular space. Proteomics analysis of cardiac ECM can be challenging due to the presence of more abundant intracellular proteins, the low degree of solubility of integral ECM proteins, and the presence of abundant posttranslational modifications. Here we describe an extraction methodology based on tissue decellularization, which allows the biochemical subfractionation of extracellular proteins in cardiac tissue. These relatively low-complexity protein fractions are suitable for analysis by gel-LC-MS/MS and other proteomics techniques.

AB - Cardiacfibrosis is characterized by excessive deposition of extracellular matrix (ECM) and is a common complication of various cardiovascular diseases. However, little is known about proteins in the cardiac extracellular space. Proteomics analysis of cardiac ECM can be challenging due to the presence of more abundant intracellular proteins, the low degree of solubility of integral ECM proteins, and the presence of abundant posttranslational modifications. Here we describe an extraction methodology based on tissue decellularization, which allows the biochemical subfractionation of extracellular proteins in cardiac tissue. These relatively low-complexity protein fractions are suitable for analysis by gel-LC-MS/MS and other proteomics techniques.

KW - Cardiacfibrosis

KW - Extracellular matrix

KW - Fractionation techniques

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A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis

Abstract

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Keywords

UN SDGs

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