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A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit

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A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit. / Almohammed, E.; Zgonjanin, D.; Iyengar, A.; David, B.; Devesse, L.; Hadi, S.

In: Forensic Science International: Genetics Supplement Series, 21.09.2017.

Research output: Contribution to journalArticle

Harvard

Almohammed, E, Zgonjanin, D, Iyengar, A, David, B, Devesse, L & Hadi, S 2017, 'A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit', Forensic Science International: Genetics Supplement Series. https://doi.org/10.1016/j.fsigss.2017.09.158

APA

Almohammed, E., Zgonjanin, D., Iyengar, A., David, B., Devesse, L., & Hadi, S. (2017). A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit. Forensic Science International: Genetics Supplement Series. https://doi.org/10.1016/j.fsigss.2017.09.158

Vancouver

Almohammed E, Zgonjanin D, Iyengar A, David B, Devesse L, Hadi S. A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit. Forensic Science International: Genetics Supplement Series. 2017 Sep 21. https://doi.org/10.1016/j.fsigss.2017.09.158

Author

Almohammed, E. ; Zgonjanin, D. ; Iyengar, A. ; David, B. ; Devesse, L. ; Hadi, S. / A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit. In: Forensic Science International: Genetics Supplement Series. 2017.

Bibtex Download

@article{3da05f361fcd4d0b8f0daabcc9f56e55,
title = "A study of degraded skeletal samples using ForenSeq DNA Signature{\texttrademark} Kit",
abstract = "Recent advances in massively parallel sequencing (MPS) has become a very promising technology for massive genetic sequencing [1]. In this study Illumina ForenSeq{\texttrademark} DNA Signature Prep Kit was tested to determine if MPS offers a more comprehensive evaluation of degraded samples than the traditional fragment analysis/capillary electrophoresis based method. The Illumina{\textregistered} ForenSeq{\texttrademark} DNA Signature MPS Kit, includes 200 genetic loci [2]. The use of NGS would therefore reduce the analysis time and augment the identification of human remains. In this context we aimed to analyse the hard tissue degraded samples using Illumina{\textregistered} ForenSeq{\texttrademark} DNA Signature MPS Kit. These samples had given partial profiles with dropout at several loci with GlobalFiler{\texttrademark} kit previously. The MPS kit showed that it is highly sensitive, aids in higher allele recovery for STR loci and provides valuable information about biogeographic ancestry, identity and phenotypic features from a single analysis. The work resulted in highly successful amplification and sequencing of 30 degraded bone/teeth samples using MPS method.",
keywords = "MPS, ForenSeq{\texttrademark}, MiSeq FGX, GlobalFiler{\texttrademark}, Autosomal STR, Forensic, Qatar",
author = "E. Almohammed and D. Zgonjanin and A. Iyengar and B. David and L. Devesse and S. Hadi",
year = "2017",
month = sep,
day = "21",
doi = "10.1016/j.fsigss.2017.09.158",
language = "English",
journal = "Forensic Science International: Genetics Supplement Series",
issn = "1875-1768",
publisher = "Elsevier Ireland Ltd",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - A study of degraded skeletal samples using ForenSeq DNA Signature™ Kit

AU - Almohammed, E.

AU - Zgonjanin, D.

AU - Iyengar, A.

AU - David, B.

AU - Devesse, L.

AU - Hadi, S.

PY - 2017/9/21

Y1 - 2017/9/21

N2 - Recent advances in massively parallel sequencing (MPS) has become a very promising technology for massive genetic sequencing [1]. In this study Illumina ForenSeq™ DNA Signature Prep Kit was tested to determine if MPS offers a more comprehensive evaluation of degraded samples than the traditional fragment analysis/capillary electrophoresis based method. The Illumina® ForenSeq™ DNA Signature MPS Kit, includes 200 genetic loci [2]. The use of NGS would therefore reduce the analysis time and augment the identification of human remains. In this context we aimed to analyse the hard tissue degraded samples using Illumina® ForenSeq™ DNA Signature MPS Kit. These samples had given partial profiles with dropout at several loci with GlobalFiler™ kit previously. The MPS kit showed that it is highly sensitive, aids in higher allele recovery for STR loci and provides valuable information about biogeographic ancestry, identity and phenotypic features from a single analysis. The work resulted in highly successful amplification and sequencing of 30 degraded bone/teeth samples using MPS method.

AB - Recent advances in massively parallel sequencing (MPS) has become a very promising technology for massive genetic sequencing [1]. In this study Illumina ForenSeq™ DNA Signature Prep Kit was tested to determine if MPS offers a more comprehensive evaluation of degraded samples than the traditional fragment analysis/capillary electrophoresis based method. The Illumina® ForenSeq™ DNA Signature MPS Kit, includes 200 genetic loci [2]. The use of NGS would therefore reduce the analysis time and augment the identification of human remains. In this context we aimed to analyse the hard tissue degraded samples using Illumina® ForenSeq™ DNA Signature MPS Kit. These samples had given partial profiles with dropout at several loci with GlobalFiler™ kit previously. The MPS kit showed that it is highly sensitive, aids in higher allele recovery for STR loci and provides valuable information about biogeographic ancestry, identity and phenotypic features from a single analysis. The work resulted in highly successful amplification and sequencing of 30 degraded bone/teeth samples using MPS method.

KW - MPS

KW - ForenSeq™

KW - MiSeq FGX

KW - GlobalFiler™

KW - Autosomal STR

KW - Forensic

KW - Qatar

U2 - 10.1016/j.fsigss.2017.09.158

DO - 10.1016/j.fsigss.2017.09.158

M3 - Article

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

SN - 1875-1768

ER -

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