A transcriptional toolbox for exploring peripheral neuro-immune interactions

TY - JOUR

T1 - A transcriptional toolbox for exploring peripheral neuro-immune interactions

AU - Liang, Zhi

AU - Hore, Zoe

AU - Harley, Peter

AU - Stanley, Federico Uchenna

AU - Michrowska, Aleksandra

AU - Dahiya, Monica

AU - La Russa, Federica

AU - Jager, Sara E

AU - Villa-Hernandez, Sara

AU - Denk, Franziska

PY - 2020/6/24

Y1 - 2020/6/24

N2 - Correct communication between immune cells and peripheral neurons is crucial for the protection of our bodies. Its breakdown is observed in many common, often painful conditions, including arthritis, neuropathies and inflammatory bowel or bladder disease. Here, we have characterised the immune response in a mouse model of neuropathic pain using flow cytometry and cell-type specific RNA sequencing (RNA-seq). We found few striking sex differences, but a very persistent inflammatory response, with increased numbers of monocytes and macrophages up to 3½ months after the initial injury. This raises the question of whether the commonly used categorisation of pain into "inflammatory" and "neuropathic" is one that is mechanistically appropriate. Finally, we collated our data with other published RNA-seq datasets on neurons, macrophages and Schwann cells in naïve and nerve injury states. The result is a practical web-based tool for the transcriptional data-mining of peripheral neuroimmune interactions.

AB - Correct communication between immune cells and peripheral neurons is crucial for the protection of our bodies. Its breakdown is observed in many common, often painful conditions, including arthritis, neuropathies and inflammatory bowel or bladder disease. Here, we have characterised the immune response in a mouse model of neuropathic pain using flow cytometry and cell-type specific RNA sequencing (RNA-seq). We found few striking sex differences, but a very persistent inflammatory response, with increased numbers of monocytes and macrophages up to 3½ months after the initial injury. This raises the question of whether the commonly used categorisation of pain into "inflammatory" and "neuropathic" is one that is mechanistically appropriate. Finally, we collated our data with other published RNA-seq datasets on neurons, macrophages and Schwann cells in naïve and nerve injury states. The result is a practical web-based tool for the transcriptional data-mining of peripheral neuroimmune interactions.

U2 - https://doi.org/10.1101/813980

DO - https://doi.org/10.1101/813980

M3 - Article

C2 - 32639365

SN - 0304-3959

JO - Pain

JF - Pain

ER -