AAV2 Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome

Michael Seyffert; Daniel L Glauser; Kurt Tobler; Oleg Georgiev; Rebecca Vogel; Bernd Vogt; Leticia Agúndez; Michael Linden; Hildegard Büning; Mathias Ackermann; Cornel Fraefel

doi:10.1128/JVI.01370-15

AAV2 Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome

Michael Seyffert, Daniel L Glauser, Kurt Tobler, Oleg Georgiev, Rebecca Vogel, Bernd Vogt, Leticia Agúndez, Michael Linden, Hildegard Büning, Mathias Ackermann, Cornel Fraefel

Infectious Diseases

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Adeno-associated virus type-2 is known to inhibit replication of herpes simplex virus type-1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep-helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/78 to the HSV-1 genome.

Original language	English
Journal	Journal of Virology
DOIs	https://doi.org/10.1128/JVI.01370-15
Publication status	E-pub ahead of print - 19 Aug 2015

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10.1128/JVI.01370-15

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title = "AAV2 Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome",

abstract = "Adeno-associated virus type-2 is known to inhibit replication of herpes simplex virus type-1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep-helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/78 to the HSV-1 genome.",

author = "Michael Seyffert and Glauser, {Daniel L} and Kurt Tobler and Oleg Georgiev and Rebecca Vogel and Bernd Vogt and Leticia Ag{\'u}ndez and Michael Linden and Hildegard B{\"u}ning and Mathias Ackermann and Cornel Fraefel",

year = "2015",

month = aug,

day = "19",

doi = "10.1128/JVI.01370-15",

language = "English",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - AAV2 Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome

AU - Seyffert, Michael

AU - Glauser, Daniel L

AU - Tobler, Kurt

AU - Georgiev, Oleg

AU - Vogel, Rebecca

AU - Vogt, Bernd

AU - Agúndez, Leticia

AU - Linden, Michael

AU - Büning, Hildegard

AU - Ackermann, Mathias

AU - Fraefel, Cornel

PY - 2015/8/19

Y1 - 2015/8/19

N2 - Adeno-associated virus type-2 is known to inhibit replication of herpes simplex virus type-1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep-helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/78 to the HSV-1 genome.

AB - Adeno-associated virus type-2 is known to inhibit replication of herpes simplex virus type-1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep-helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/78 to the HSV-1 genome.

U2 - 10.1128/JVI.01370-15

DO - 10.1128/JVI.01370-15

M3 - Article

C2 - 26292324

SN - 0022-538X

JO - Journal of Virology

JF - Journal of Virology

ER -

AAV2 Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome

Abstract

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