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Accelerating localisation microscopy

Research output: Chapter in Book/Report/Conference proceedingConference paperpeer-review

Original languageEnglish
Title of host publicationCLEO: Science and Innovations, CLEO-SI 2015
PublisherOptical Society of America (OSA)
Pages2267
Number of pages1
ISBN (Print)9781557529688
DOIs
Published4 May 2015
EventCLEO: Science and Innovations, CLEO-SI 2015 - San Jose, United States
Duration: 10 May 201515 May 2015

Conference

ConferenceCLEO: Science and Innovations, CLEO-SI 2015
Country/TerritoryUnited States
CitySan Jose
Period10/05/201515/05/2015

King's Authors

Abstract

Fluorescence localisation microscopy is a powerful tool for imaging cell structures at a lengthscale of tens of nm, but its utility for live cell imaging is limited, as it takes a long time to acquire the data needed for a super-resolution image. However, the data acquisition time can be cut by more than two orders of magnitude by using advanced algorithms which can analyse dense data. Different algorithms offer tradeoffs between acquisition and processing time, and quality of the fitted data.

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