Activity-dependent regulation of inhibitory synaptic transmission in hippocampal neurons.

Kenichi N Hartman; Sumon K Pal; Juan Burrone; Venkatesh Murthy

doi:10.1038/nn1677

Activity-dependent regulation of inhibitory synaptic transmission in hippocampal neurons.

Kenichi N Hartman, Sumon K Pal, Juan Burrone, Venkatesh Murthy

Developmental Neurobiology

Research output: Contribution to journal › Article › peer-review

176 Citations (Scopus)

Abstract

Neural activity regulates the number and properties of GABAergic synapses in the brain, but the mechanisms underlying these changes are unclear. We found that blocking spike activity globally in developing hippocampal neurons from rats reduced the density of GABAergic terminals as well as the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs). Chronic inactivity later in development led to a reduction in the mIPSC amplitude, without any change in GABAergic synapse density. By contrast, hyperpolarizing or abolishing spike activity in single neurons did not alter GABAergic synaptic inputs. Suppressing activity in individual presynaptic GABAergic neurons also failed to decrease synaptic output. Our results indicate that GABAergic synapses are regulated by the level of activity in surrounding neurons. Notably, we found that the expression of GABAergic plasticity involves changes in the amount of neurotransmitter in individual vesicles.

Original language	English
Pages (from-to)	642 - 649
Number of pages	8
Journal	Nature Neuroscience
Volume	9
Issue number	5
DOIs	https://doi.org/10.1038/nn1677
Publication status	Published - May 2006

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title = "Activity-dependent regulation of inhibitory synaptic transmission in hippocampal neurons.",

abstract = "Neural activity regulates the number and properties of GABAergic synapses in the brain, but the mechanisms underlying these changes are unclear. We found that blocking spike activity globally in developing hippocampal neurons from rats reduced the density of GABAergic terminals as well as the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs). Chronic inactivity later in development led to a reduction in the mIPSC amplitude, without any change in GABAergic synapse density. By contrast, hyperpolarizing or abolishing spike activity in single neurons did not alter GABAergic synaptic inputs. Suppressing activity in individual presynaptic GABAergic neurons also failed to decrease synaptic output. Our results indicate that GABAergic synapses are regulated by the level of activity in surrounding neurons. Notably, we found that the expression of GABAergic plasticity involves changes in the amount of neurotransmitter in individual vesicles.",

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AU - Pal, Sumon K

AU - Burrone, Juan

AU - Murthy, Venkatesh

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Activity-dependent regulation of inhibitory synaptic transmission in hippocampal neurons.

Abstract

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