Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice

TY - JOUR

T1 - Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice

AU - Fantozzi, Evelyn Thais

AU - Garbin, Sara

AU - Yamamoto-Ricardo-da-Silva, Fernanda

AU - Oliveira-Filho, Ricardo Martins

AU - Spina, Domenico

AU - Tavares-de-Lima, Wothan

AU - Riffo-Vasquez, Yanira

N1 - Copyright © 2018. Published by Elsevier Ltd.

PY - 2018/4

Y1 - 2018/4

N2 - RATIONAL: Acute lung injury (ALI) is a common complication after intestinal ischemia and reperfusion (I/R) injury that can lead to acute respiratory distress syndrome (ARDS). We have previously demonstrated that females are protected against lung damage induced by intestinal I/R through an estrogen mediated mechanism.OBJECTIVES: to investigate the effect of obesity on ALI induced by intestinal I/R in female mice.METHODS: C57Bl/6 female mice were fed with a standard low-fat diet (SD) or a high-fat diet (HFD) for 9 weeks. Intestinal I/R injury was induced by a 45 min occlusion of the mesenteric artery followed by 2 and 24 h of reperfusion.RESULTS: Significant increase in lung myeloperoxidase expression (MPO) and neutrophil numbers of SD and HFD mice occurred at 2 h and 24 h of reperfusion. Furthermore, HFD mice presented a significant increase in lung eosinophil peroxidase (EPO) expression and eosinophil numbers compared to SD mice. Lung wet/dry weight ratio was significantly greater in HFD mice at 2 and 24 h of reperfusion, accompanied by a significant increase in the expression of inducible NO in the lung tissue and a significant decrease in arterial oxygen saturation at 24 h of reperfusion relative to SD mice.CONCLUSION: Obesity predisposes female mice to increased pulmonary oedema and deterioration in gas exchange, which is accompanied by an increase in iNOS expression in the lung.

AB - RATIONAL: Acute lung injury (ALI) is a common complication after intestinal ischemia and reperfusion (I/R) injury that can lead to acute respiratory distress syndrome (ARDS). We have previously demonstrated that females are protected against lung damage induced by intestinal I/R through an estrogen mediated mechanism.OBJECTIVES: to investigate the effect of obesity on ALI induced by intestinal I/R in female mice.METHODS: C57Bl/6 female mice were fed with a standard low-fat diet (SD) or a high-fat diet (HFD) for 9 weeks. Intestinal I/R injury was induced by a 45 min occlusion of the mesenteric artery followed by 2 and 24 h of reperfusion.RESULTS: Significant increase in lung myeloperoxidase expression (MPO) and neutrophil numbers of SD and HFD mice occurred at 2 h and 24 h of reperfusion. Furthermore, HFD mice presented a significant increase in lung eosinophil peroxidase (EPO) expression and eosinophil numbers compared to SD mice. Lung wet/dry weight ratio was significantly greater in HFD mice at 2 and 24 h of reperfusion, accompanied by a significant increase in the expression of inducible NO in the lung tissue and a significant decrease in arterial oxygen saturation at 24 h of reperfusion relative to SD mice.CONCLUSION: Obesity predisposes female mice to increased pulmonary oedema and deterioration in gas exchange, which is accompanied by an increase in iNOS expression in the lung.

KW - Obesity

KW - Estrogen

KW - Lung injury

KW - Intestinal ischemia and reperfusion

KW - Inflammation

KW - Female mice

U2 - 10.1016/j.pupt.2018.01.005

DO - 10.1016/j.pupt.2018.01.005

M3 - Article

C2 - 29337267

SN - 1094-5539

VL - 49

SP - 54

EP - 59

JO - Pulmonary pharmacology & therapeutics

JF - Pulmonary pharmacology & therapeutics

ER -