Adaptive optics for time-resolved Förster Resonance Energy Transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) in vivo

TY - JOUR

T1 - Adaptive optics for time-resolved Förster Resonance Energy Transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) in vivo

AU - Ameer-Beg, Simon

AU - Poland, Simon Peter

AU - Devauges, Viviane Julie Renee Emilie

PY - 2020/2/14

Y1 - 2020/2/14

N2 - Förster Resonance Energy Transfer (FRET) and Fluorescence lifetime imaging (FLIM) have been coupled with multiphoton microscopy to image in vivo dynamics. However, the increase in optical aberrations as a function of depth significantly reduces the fluorescent signal, reduces spatial resolution and fluorescence lifetime accuracy. We present the development of time-resolved FRET-FLIM imaging system with adaptive optics. We demonstrate the improvement of our AO-FRET-FLIM instrument over standard multiphoton FRET-FLIM imaging. We validate our approach using fixed cellular samples with FRET standards and in vivo with live imaging in a mouse kidney.

AB - Förster Resonance Energy Transfer (FRET) and Fluorescence lifetime imaging (FLIM) have been coupled with multiphoton microscopy to image in vivo dynamics. However, the increase in optical aberrations as a function of depth significantly reduces the fluorescent signal, reduces spatial resolution and fluorescence lifetime accuracy. We present the development of time-resolved FRET-FLIM imaging system with adaptive optics. We demonstrate the improvement of our AO-FRET-FLIM instrument over standard multiphoton FRET-FLIM imaging. We validate our approach using fixed cellular samples with FRET standards and in vivo with live imaging in a mouse kidney.

M3 - Article

SN - 0146-9592

JO - Optics Letters

JF - Optics Letters

ER -