Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering

Ana Angelova; M. Kawasaki; P. T. Sharpe

doi:10.1177/0022034513481041

Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering

Ana Angelova, M. Kawasaki, P. T. Sharpe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

Original language	English
Pages (from-to)	329-334
Number of pages	6
Journal	Journal of Dental Research
Volume	92
Issue number	4
DOIs	https://doi.org/10.1177/0022034513481041
Publication status	Published - Apr 2013

Keywords

EMBRYO
tissue engineering
ameloblast
INDUCTION
root formation
oral epithelium
biotooth
1-YEAR
MOUSE
TEETH
MARGINAL BONE LEVEL
MALASSEZ
DENTAL IMPLANTS
MESENCHYME
RESORPTION

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title = "Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering",

abstract = "Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.",

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author = "Ana Angelova and M. Kawasaki and Sharpe, {P. T.}",

year = "2013",

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doi = "10.1177/0022034513481041",

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volume = "92",

pages = "329--334",

journal = "Journal of Dental Research",

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TY - JOUR

T1 - Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering

AU - Angelova, Ana

AU - Kawasaki, M.

AU - Sharpe, P. T.

PY - 2013/4

Y1 - 2013/4

N2 - Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

AB - Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

KW - EMBRYO

KW - tissue engineering

KW - ameloblast

KW - INDUCTION

KW - root formation

KW - oral epithelium

KW - biotooth

KW - 1-YEAR

KW - MOUSE

KW - TEETH

KW - MARGINAL BONE LEVEL

KW - MALASSEZ

KW - DENTAL IMPLANTS

KW - MESENCHYME

KW - RESORPTION

U2 - 10.1177/0022034513481041

DO - 10.1177/0022034513481041

M3 - Article

SN - 0022-0345

VL - 92

SP - 329

EP - 334

JO - Journal of Dental Research

JF - Journal of Dental Research

IS - 4

ER -

Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering

Abstract

Keywords

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