Ageing modulates human dermal fibroblast contractility: quantification using nano-biomechanical testing

Zhuonan Yu, Matthew Smith, Richard Siow, Kuo-Kang Liu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
35 Downloads (Pure)


Dermal fibroblasts play a key role in maintaining homoeostasis and functionality of the skin. Their contractility plays a role in changes observed during ageing, especially in processes such as wound healing, inflammation, wrinkling and scar tissue formation as well as structural changes on extracellular matrix. Although alternations in skin physiology and morphology have been previously described, there remains a paucity of information about the influence of chronological ageing on dermal fibroblast contractility. In this study, we applied a novel nano-biomechanical technique on cell-embedded collagen hydrogels in combination with mathematical modelling and numerical simulation to measure contraction forces of normal human dermal fibroblasts (NHDF). We achieved quantitative differentiation of the contractility of cells derived from ‘young’ (< 30 years old) and ‘aged’ (> 60 years old) donors. Transforming growth factor β1 (TGF-β1) was used to stimulate the fibroblasts to assess their contractile potential. NHDF from aged donors exhibited a greater basal contractile force, while in contrast, NHDF from young donors have shown a significantly larger contractile force in response to TGF-β1 treatment. These findings validate our nano-biomechanical measurement technique and provide new insights for considering NHDF contractility in regenerative medicine and as a biomarker of dermal ageing processes.
Original languageEnglish
Article number118972
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number5
Early online date27 Jan 2021
Publication statusPublished - 5 Apr 2021


  • Ageing
  • Cell mechanics
  • Nanoindentation
  • Force displacement
  • TGF-β1
  • Finite element


Dive into the research topics of 'Ageing modulates human dermal fibroblast contractility: quantification using nano-biomechanical testing'. Together they form a unique fingerprint.

Cite this