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Alcoholic and non-alcoholic beer modulate plasma and macrophage micrornas differently in a pilot intervention in humans with cardiovascular risk

Research output: Contribution to journalArticlepeer-review

Lidia Daimiel, Víctor Micó, Laura Díez-Ricote, Paloma Ruiz-Valderrey, Geoffrey Istas, Ana Rodríguez-Mateos, José María Ordovás

Original languageEnglish
Article number69
Pages (from-to)1-21
Number of pages21
JournalNutrients
Volume13
Issue number1
DOIs
PublishedJan 2021

Bibliographical note

Funding Information: The presented work was funded by Fundaci?n Salud 2000?Merck Serono research fellowship granted to JMO, by the official funding agency for biomedical research of the Spanish govern-ment, ISCIII, through the Fondo de Investigaci?n para la Salud (FIS) granted to LD (PI14/01374 and PI17/00508), which was co-funded by the European Regional Development Fund and by the COST Action FA1403?POSITIVe ?Interindividual variation in response to consumption of plant food bioactives and determinants involved? supported by COST (European Cooperation in Science and Technology, http://www.cost.eu/). VM was supported by a Manuel de Oya fellowship of the Fundaci?n Cerveza y Salud. None of the funding sources took part in the design, collection, analysis, or interpretation of the data, or in the decision to submit the manuscript for publication. Acknowledgments: The authors thanks miRoBeer participants for their enthusiastic collaboration. Graphical abstract contains elements of Servier Medical Art, reproduced under CC BY license. Funding Information: Funding: The presented work was funded by Fundación Salud 2000–Merck Serono research fellowship granted to JMO, by the official funding agency for biomedical research of the Spanish government, ISCIII, through the Fondo de Investigación para la Salud (FIS) granted to LD (PI14/01374 and PI17/00508), which was co-funded by the European Regional Development Fund and by the COST Action FA1403—POSITIVe “Interindividual variation in response to consumption of plant food bioactives and determinants involved” supported by COST (European Cooperation in Science and Technology, http://www.cost.eu/). VM was supported by a Manuel de Oya fellowship of the Fundación Cerveza y Salud. None of the funding sources took part in the design, collection, analysis, or interpretation of the data, or in the decision to submit the manuscript for publication. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors

Abstract

Beer is a popular beverage and some beneficial effects have been attributed to its moderate consumption. We carried out a pilot study to test if beer and non-alcoholic beer consumption modify the levels of a panel of 53 cardiometabolic microRNAs in plasma and macrophages. Seven non-smoker men aged 30–65 with high cardiovascular risk were recruited for a non-randomised cross-over intervention consisting of the ingestion of 500 mL/day of beer or non-alcoholic beer for 14 days with a 7-day washout period between interventions. Plasma and urine isoxanthohumol were measured to assess compliance with interventions. Monocytes were isolated and differentiated into macrophages, and plasma and macrophage microRNAs were analysed by quantitative real-time PCR. Anthropometric, biochemistry and dietary parameters were also measured. We found an increase in plasma miR-155-5p, miR-328-3p, and miR-92a-3p after beer and a decrease after non-alcoholic beer consumption. Plasma miR-320a-3p levels decreased with both beers. Circulating miR-320a-3p levels correlated with LDL-cholesterol. We found that miR-17-5p, miR-20a-5p, miR-145-5p, miR-26b-5p, and miR-223-3p macrophage levels increased after beer and decreased after non-alcoholic beer consumption. Functional analyses suggested that modulated microRNAs were involved in catabolism, nutrient sensing, Toll-like receptors signalling and inflammation. We concluded that beer and non-alcoholic beer intake modulated differentially plasma and macrophage microRNAs. Specifically, microRNAs related to inflammation increased after beer consumption and decreased after non-alcoholic beer consumption.

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