Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses

Chris J. Corrigan, Wei Wang, Qiu Meng, Cailong Fang, Ghada Eid, M. Rosario Caballero, Ze Lv, Yunqing An, Yui-Hsi Wang, Yong-Jun Liu, A. Barry Kay, Tak H. Lee, Ying Sun

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    172 Citations (Scopus)

    Abstract

    Background: IL-25 is thought to participate in allergic inflammation by propagating T(H)2-type responses. Objective: To address the hypothesis that allergen provocation increases expression of IL-25 and its receptor IL-25R in the asthmatic bronchial mucosa and skin dermis of atopic subjects. Methods: Sequential single and double immunostaining was used to evaluate the numbers and phenotypes of IL-25 and IL-25R immunoreactive cells in bronchial biopsies from mild atopic subjects with asthma (n = 10) before and 24 hours after allergen inhalation challenge and skin biopsies from atopic subjects (n = 10) up to 72 hours after allergen subepidermal injection. Results: IL-25 immunoreactivity was expressed by a majority of epidermal cells in both organs at baseline and was not further augmented by challenge. IL-25R immunoreactive cells were rare in the epidermis before or after challenge. Allergen challenge was associated with significantly (P <.01) increased expression of IL-25 and IL-25R immunoreactivity in the submucosa of both organs. IL-25 immunoreactivity colocalized with eosinophils, mast cells, and endothelial cells, whereas IL-25R immunoreactivity colocalized with eosinophils, mast cells, endothelial cells, and T lymphocytes. In both organs, correlations were observed between increases in IL-25 expression and the magnitudes of the late-phase allergen-induced clinical responses. Conclusion: Allergen provocation induces functionally relevant, increased expression of IL-25 and its receptor in the asthmatic bronchial mucosa and dermis of sensitized atopic subjects. In addition to T cells, eosinophils, mast cells, and endothelial cells are potential sources and targets of IL-25 in the course of allergic inflammation. (J Allergy Clin Immunol 2011; 128: 116-24.)
    Original languageEnglish
    Pages (from-to)116 - 124
    Number of pages9
    JournalJournal of Allergy and Clinical Immunology
    Volume128
    Issue number1
    DOIs
    Publication statusPublished - Jul 2011

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